Assessment of  a Maslinic Acid Derivative and its Metabolite in Rat Blood by Liquid Chromatography Coupled with Mass Spectrometry

Pavel, Ioana Zinuca; Dehelean, Cristina Adriana; Farczadi, Lenard; Muntean, Dana Maria; Vlase, Laurian; Danciu, Corina; Csuk, Rene; Birsasteanu, Florin; Muntean, Danina Mirela

Ioana Zinuca Pavel, Cristina Adriana Dehelean, Lenard Farczadi, Dana Maria Muntean, Laurian Vlase, Corina Danciu, Rene Csuk, Florin Birsasteanu, Danina Mirela Muntean

Assessment of a Maslinic Acid Derivative and its Metabolite in Rat Blood by Liquid Chromatography Coupled with Mass Spectrometry

Abstract:

A new rapid high-throughput liquid chromatography coupled with tandem mass spectrometry method (LC-MS/MS) was developed for the blood assessment of a maslinic acid derivative (Benzyl (2a, 3b) 2,3-diacetoxy-olean-12-en-28-amide) and its metabolite. Separation was performed using a Zorbax SB-C18 column with a mobile phase composition of methanol and 0.2% formic acid in water (78:22, v/v) in isocratic flow, with a flow rate of 0.6 mL/min. Detection was performed by multiple reaction monitoring (MRM) mode, monitoring the transitions 338.3; 526.6; 544.6; 527.5; 545.5 m/z derived from 646.6 m/z for the maslinic acid derivative and 409; 338.3; 203; 205; 526.5 m/z derived from 562.6 m/z for its metabolite, using atmospheric pressure chemical positive ionisation source. Single-step protein precipitation with methanol was used for preparation of rat blood samples. The method was validated with respect to selectivity, linearity (r ] 0.9937), within-run and between-run precision (CV [ 12.0%) and accuracy (bias [ 7.9%) over the ranges of 20.5 - 656 ng/mL blood for maslinic acid derivative, and 20 - 640 ng/mL blood for maslinic acid derivative metabolite. The analytical method is simple, fast, selective and is suitable for bioanalysis of maslinic acid derivative and its metabolite from rat blood.

Keywords:
LC/MS analysis; maslinic acid derivative; metabolite; rat blood

Issue: 2017, Volume 68, Issue 5

Pages: 1089-1094

Publication date: 2017/6/15

https://doi.org/10.37358/RC.17.5.5618

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This article is published under the Creative Commons Attribution 4.0 International License

Citation Styles

Cite this article as:
PAVEL, I.Z., DEHELEAN, C.A., FARCZADI, L., MUNTEAN, D.M., VLASE, L., DANCIU, C., CSUK, R., BIRSASTEANU, F., MUNTEAN, D.M., Assessment of a Maslinic Acid Derivative and its Metabolite in Rat Blood by Liquid Chromatography Coupled with Mass Spectrometry, Rev. Chim., 68(5), 2017, 1089-1094.

Vancouver
Pavel IZ, Dehelean CA, Farczadi L, Muntean DM, Vlase L, Danciu C, Csuk R, Birsasteanu F, Muntean DM. Assessment of a Maslinic Acid Derivative and its Metabolite in Rat Blood by Liquid Chromatography Coupled with Mass Spectrometry. Rev. Chim.[internet]. 2017 May;68(5):1089-1094. Available from: https://doi.org/10.37358/RC.17.5.5618

APA 6th edition
Pavel, I.Z., Dehelean, C.A., Farczadi, L., Muntean, D.M., Vlase, L., Danciu, C.,... Muntean, D.M. (2017). Assessment of a Maslinic Acid Derivative and its Metabolite in Rat Blood by Liquid Chromatography Coupled with Mass Spectrometry. Revista de Chimie, 68(5), 1089-1094. https://doi.org/10.37358/RC.17.5.5618

Harvard
Pavel, I.Z., Dehelean, C.A., Farczadi, L., Muntean, D.M., Vlase, L., Danciu, C., Csuk, R., Birsasteanu, F., Muntean, D.M. (2017). 'Assessment of a Maslinic Acid Derivative and its Metabolite in Rat Blood by Liquid Chromatography Coupled with Mass Spectrometry', Revista de Chimie, 68(5), pp. 1089-1094. https://doi.org/10.37358/RC.17.5.5618

IEEE
I.Z. Pavel, C.A. Dehelean, L. Farczadi, D.M. Muntean, L. Vlase, C. Danciu, R. Csuk, F. Birsasteanu, D.M. Muntean, "Assessment of a Maslinic Acid Derivative and its Metabolite in Rat Blood by Liquid Chromatography Coupled with Mass Spectrometry". Revista de Chimie, vol. 68, no. 5, pp. 1089-1094, 2017. [online]. https://doi.org/10.37358/RC.17.5.5618

Text
Ioana Zinuca Pavel, Cristina Adriana Dehelean, Lenard Farczadi, Dana Maria Muntean, Laurian Vlase, Corina Danciu, Rene Csuk, Florin Birsasteanu, Danina Mirela Muntean,
Assessment of a Maslinic Acid Derivative and its Metabolite in Rat Blood by Liquid Chromatography Coupled with Mass Spectrometry,
Revista de Chimie,
Volume 68, Issue 5,
2017,
Pages 1089-1094,
ISSN 2668-8212,
https://doi.org/10.37358/RC.17.5.5618.
(https://revistadechimie.ro/Articles.asp?ID=5618)
Keywords: LC/MS analysis; maslinic acid derivative; metabolite; rat blood

RIS
TY - JOUR
T1 - Assessment of a Maslinic Acid Derivative and its Metabolite in Rat Blood by Liquid Chromatography Coupled with Mass Spectrometry
A1 - Pavel, Ioana Zinuca
A2 - Dehelean, Cristina Adriana
A3 - Farczadi, Lenard
A4 - Muntean, Dana Maria
A5 - Vlase, Laurian
A6 - Danciu, Corina
A7 - Csuk, Rene
A8 - Birsasteanu, Florin
A9 - Muntean, Danina Mirela
JF - Revista de Chimie
JO - Rev. Chim.
PB - Revista de Chimie SRL
SN - 2668-8212
Y1 - 2017
VL - 68
IS - 5
SP - 1089
EP - 1094
UR - https://doi.org/10.37358/RC.17.5.5618
KW - LC/MS analysis
KW - maslinic acid derivative
KW - metabolite
KW - rat blood
ER -

BibTex
@article{RevCh2017P1089,
author = {Pavel Ioana Zinuca and Dehelean Cristina Adriana and Farczadi Lenard and Muntean Dana Maria and Vlase Laurian and Danciu Corina and Csuk Rene and Birsasteanu Florin and Muntean Danina Mirela},
title = {Assessment of a Maslinic Acid Derivative and its Metabolite in Rat Blood by Liquid Chromatography Coupled with Mass Spectrometry},
journal = {Revista de Chimie},
volume = {68},
number = {5},
pages = {1089-1094},
year = {2017},
issn = {2668-8212},
doi = {https://doi.org/10.37358/RC.17.5.5618},
url = {https://revistadechimie.ro/Articles.asp?ID=5618}
}

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