In vitro Three Dimensional Scaffold-free Construct of Human Adipose-derived Stem Cells in Coculture with Endothelial Cells and Fibroblasts

Berea, Grigore; Balan, Gheorghe Gh.; Sandru, Vasile; Sirbu, Paul Dan

Grigore Berea, Gheorghe Gh. Balan, Vasile Sandru, Paul Dan Sirbu

In vitro Three Dimensional Scaffold-free Construct of Human Adipose-derived Stem Cells in Coculture with Endothelial Cells and Fibroblasts

Abstract:

Complex interactions between stem cells, vascular cells and fibroblasts represent the substrate of building microenvironment-embedded 3D structures that can be grafted or added to bone substitute scaffolds in tissue engineering or clinical bone repair. Human Adipose-derived Stem Cells (hASCs), human umbilical vein endothelial cells (HUVECs) and normal dermal human fibroblasts (NDHF) can be mixed together in three dimensional scaffold free constructs and their behaviour will emphasize their potential use as seeding points in bone tissue engineering. Various combinations of the aforementioned cell lines were compared to single cell line culture in terms of size, viability and cell proliferation. At 5 weeks, viability dropped for single cell line spheroids while addition of NDHF to hASC maintained the viability at the same level at 5 weeks Fibroblasts addition to the 3D construct of stem cells and endothelial cells improves viability and reduces proliferation as a marker of cell differentiation toward osteogenic line.

Keywords:
microenvironment-embedded 3D structures; tissue engineering; Stem and endothelial cells; viability and cell proliferation; ascorbic acid; hydrocortisone; Na-pyruvate L-Glutamine

Issue: 2017, Volume 68, Issue 6

Pages: 1341-1344

Publication date: 2017/7/15

https://doi.org/10.37358/RC.17.6.5670

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This article is published under the Creative Commons Attribution 4.0 International License

Citation Styles

Cite this article as:
BEREA, G., BALAN, G.G., SANDRU, V., SIRBU, P.D., In vitro Three Dimensional Scaffold-free Construct of Human Adipose-derived Stem Cells in Coculture with Endothelial Cells and Fibroblasts, Rev. Chim., 68(6), 2017, 1341-1344.

Vancouver
Berea G, Balan GG, Sandru V, Sirbu PD. In vitro Three Dimensional Scaffold-free Construct of Human Adipose-derived Stem Cells in Coculture with Endothelial Cells and Fibroblasts. Rev. Chim.[internet]. 2017 Jun;68(6):1341-1344. Available from: https://doi.org/10.37358/RC.17.6.5670

APA 6th edition
Berea, G., Balan, G.G., Sandru, V. & Sirbu, P.D. (2017). In vitro Three Dimensional Scaffold-free Construct of Human Adipose-derived Stem Cells in Coculture with Endothelial Cells and Fibroblasts. Revista de Chimie, 68(6), 1341-1344. https://doi.org/10.37358/RC.17.6.5670

Harvard
Berea, G., Balan, G.G., Sandru, V., Sirbu, P.D. (2017). 'In vitro Three Dimensional Scaffold-free Construct of Human Adipose-derived Stem Cells in Coculture with Endothelial Cells and Fibroblasts', Revista de Chimie, 68(6), pp. 1341-1344. https://doi.org/10.37358/RC.17.6.5670

IEEE
G. Berea, G.G. Balan, V. Sandru, P.D. Sirbu, "In vitro Three Dimensional Scaffold-free Construct of Human Adipose-derived Stem Cells in Coculture with Endothelial Cells and Fibroblasts". Revista de Chimie, vol. 68, no. 6, pp. 1341-1344, 2017. [online]. https://doi.org/10.37358/RC.17.6.5670

Text
Grigore Berea, Gheorghe Gh. Balan, Vasile Sandru, Paul Dan Sirbu,
In vitro Three Dimensional Scaffold-free Construct of Human Adipose-derived Stem Cells in Coculture with Endothelial Cells and Fibroblasts,
Revista de Chimie,
Volume 68, Issue 6,
2017,
Pages 1341-1344,
ISSN 2668-8212,
https://doi.org/10.37358/RC.17.6.5670.
(https://revistadechimie.ro/Articles.asp?ID=5670)
Keywords: microenvironment-embedded 3D structures; tissue engineering; Stem and endothelial cells; viability and cell proliferation; ascorbic acid; hydrocortisone; Na-pyruvate L-Glutamine

RIS
TY - JOUR
T1 - In vitro Three Dimensional Scaffold-free Construct of Human Adipose-derived Stem Cells in Coculture with Endothelial Cells and Fibroblasts
A1 - Berea, Grigore
A2 - Balan, Gheorghe Gh.
A3 - Sandru, Vasile
A4 - Sirbu, Paul Dan
JF - Revista de Chimie
JO - Rev. Chim.
PB - Revista de Chimie SRL
SN - 2668-8212
Y1 - 2017
VL - 68
IS - 6
SP - 1341
EP - 1344
UR - https://doi.org/10.37358/RC.17.6.5670
KW - microenvironment-embedded 3D structures
KW - tissue engineering
KW - Stem and endothelial cells
KW - viability and cell proliferation
KW - ascorbic acid
KW - hydrocortisone
KW - Na-pyruvate L-Glutamine
ER -

BibTex
@article{RevCh2017P1341,
author = {Berea Grigore and Balan Gheorghe Gh. and Sandru Vasile and Sirbu Paul Dan},
title = {In vitro Three Dimensional Scaffold-free Construct of Human Adipose-derived Stem Cells in Coculture with Endothelial Cells and Fibroblasts},
journal = {Revista de Chimie},
volume = {68},
number = {6},
pages = {1341-1344},
year = {2017},
issn = {2668-8212},
doi = {https://doi.org/10.37358/RC.17.6.5670},
url = {https://revistadechimie.ro/Articles.asp?ID=5670}
}

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