ADELA CRISTINA LAZAR, RADU LACATUS, ROBERT PURDOIU, LUCIA BEL, ORSOLYA SARPATAKI, IONEL PAPUC, MARIANA PACURAR, RADU SEPTIMIU CAMPIAN IMPLICATIONS OF CREATININE AND ALP IN THE INDUCTION OF OSTEOPOROSIS BY OVARIECTOMY IN FEMALE WISTAR RATS Histological and biochemical assessment of maxillary bone necrosis induced by therapy with two bisphosphonate types used to treat osteoporosis induced in female Wistar rats, evaluation of effects on creatinine and ALP values at different time intervals. Female Wistar rats were ovariectomized to induce osteoporosis. Blood tests for ALP and creatinine were conducted. They received two types of bisphosphonates. Dental extraction was performed in each rat separately; finally, the rats were euthanized. Microscopic evaluation of the tissue fragments collected from the groups of rats was performed for the following parameters: degree of osteonecrosis, bone resorption, inflammatory infiltrate with neutrophils and mononuclear cells, number of osteoclasts, degree of proliferation of fibrous connective tissue, and local neovascularization. The biochemical and subsequently histological investigations for osteonecrosis of the jaw associated with bisphosphonate therapy in rats with induced osteoporosis provide accurate information about the extent of osteonecrosis, bone resorption, inflammatory infiltrate with mononuclear cells and neutrophils, number of osteoclasts, degree of proliferation of fibrous connective tissue, and local neovascularization. This is necessary for positive and differential diagnosis and for the treatment plan.
Keywords: alkaline phosphatase, creatinine, osteoporosis, rats, bone necrosis