CARMEN GENTIMIR, DIANA TATARCIUC, CATALINA ANDA ZAHARIA, LILIANA CHELARU, ANDREEA COSTIN, ALEXANDRA JITAREANU, COSTICA BEJINARIU, MARCEL COSTULEANU BIOCHEMICAL EFFECTS OF SOME TYROSINE KINASE INHIBITORS ON PRO-B CELLS-INDUCED APOPTOSIS The present studies aimed the effects of tyrphostin AG 494 and tyrphostin AG 1295 on apoptosis of mouse pro-B lymphocytes. The actual scientific literature lacks such data. Tyrphostin AG 494 is an inhibitor of epidermal growth factor receptor pathways and tyrphostin AG 1295 is an inhibitor of platelet-derived growth factor receptor pathways. Our obtained data demonstrated that tyrphostin AG 1295 was less effective in preventing the apoptosis of murine pro-B cells, triggered by the combination of Cytoporone B (NR4A1 agonist) and Cyclosporine A. In contrast, tyrphostin AG 494 had a stronger inhibitory effect on the apoptosis of the same cells, when administered for 24 hours. Thus, when blocking the activation of epidermal growth factor receptor pathways, the inductive apoptotic effects of Cytoporone B and Cyclosporine A are reduced. Thus, we could conclude that such inhibition will increase the resistance to apoptosis of pro-B cells. Thus, such a resistance to apoptosis could be experimentally acquired by hematopoietic cells.
Keywords: tyrphostin AG 494, tyrphostin AG 1295, pro-B cells, apoptosis