IOANA MARTU, ANCUTA GORIUC, MARIA ALEXANDRA MARTU, IOANA VATA, RALUCA BACIU, RALUCA MOCANU, AMELIA ELENA SURDU, COSMIN POPA, IONUT LUCHIAN IDENTIFICATION OF BACTERIA INVOLVED IN PERIODONTAL DISEASE USING MOLECULAR BIOLOGY TECHNIQUES Periodontal disease is progressive and episodic in nature, with tissue destruction resulting from the host response to bacterial antigens and irritants.This study aimed to evaluate the genotype of a certain group of pathogen agents. In order to to this we used a total of 45 patients with PAG included in the epidemiological analyzes with a mean age of 34.7 Four samples were collected from the subgingival plaque from each patient, a total of 224 samples of dental plaques were investigated. All samples were tested for the presence of Tannerella forsythensis, Porphyromonas gingivalis and Treponema denticola. We found that patients had a large number of pockets colonized by Tannerella forsythensis gingivalis (88.6%) Porphyromonas gingivalis (59%) and Aggregatibacter actinomycetemcomitans (25%). The data of this study support the evidence Tannerella forsythensis as a periodontopatogen and confirmed a strong association between Porphyromonas gingivalis and Tannerella forsythensis in aggressive periodontitis and support previous findings that generalized aggressive periodontitis are associated with more complex microbiota.
Keywords: periodontal disease, biofilm, DNA hybridization