CRISTINA POPA, CARMEN GABRIELA STELEA, ANA MARIA FILIOREANU, IRINA GEORGETA SUFARU, GEORGE ALEXANDRU MAFTEI, MANUELA ARBUNE, SILVIA MARTU, EUGENIA POPESCU PCR ANALYSIS OF THE HERPESVIRUSES PRESENCE IN CREVICULAR FLUID IN HIV- POSITIVE PATIENTS
The present study aimed to determine the frequency of herpesviruses in gingival fluid samples in patients with periodontitis HIV compared to HIV-negative subjects. Gingival crevicular fluid samples were obtained from 28 patients with HIV-positive periodontitis and from 14 patients with HIV seronegative periodontitis. Herpesviruses have been identified by PCR amplification methods. In HIV-positive patients, the most prevalent herpes virus was HCMV, followed by HHV-6 and HHV-7. In non-HIV-related periodontitis, HCMV was identified in 11 samples and EBV-1 in 8 samples, followed by HSV (7 samples). HIV seropositive samples showed an average of 4.0 herpesviruses and HIV-seronegative individuals averaging 1.4 herpesviruses. EBV-2 and HHV-8 were detected exclusively in subgingival samples from HIV-positive patients. HIV-induced activation of herpes viruses may be a stimulating factor for rapid periodontal destruction. Patients with severe immunosuppression may experience herpesvirus-mediated gingival necrosis. The hypothesis that HIV periodontitis is the result of a combined infection of herpesviruses and bacterial pathogens should be studied further.