BOGDAN MIHAIL DIACONESCU, DANIELA JITARU, MIHAIELA LOREDANA DRAGOS, MAGDA BADESCU, TEODOR STEFANACHE, MANUELA CIOCOIU, MADALINA MOCANU, CODRUTA BADESCU THE EFFECT OF SOME NATURAL CYTOTOXIC PEPTIDES ON TUMOR CELLS Many studies have highlighted the anti-tumor properties of some natural peptides known to have antimicrobial virtues. In this study, we evaluated the tumoricidal potential of dermaseptin, defensin, cecropin A and B on tumor cell lines: M14K (human mesothelioma). The viability study was performed using PE V Annexin and 7-AAD (7-amino-actinomycin D) (BD Pharmingen). This test was used to detect and measure apoptosis by flow cytometry technique. The experimental results of our study revealed that the cytotoxic effects of the four peptides depend on their concentration. In this in vitro experimental study, we found that the cytotoxic effect of the four cytotoxic peptides used depended on their concentration in the tumor cell culture medium, being significant at concentrations of 120mM and maintained at concentrations of 60ìM. At 30ìM concentrations these tumoricidal effects were insignificant. Of all the studied peptides, dermaseptin has the most powerful effect and the weakest effect b - defensin - 1.
Keywords: dermaseptin, defensin, cecropin, human mesothelioma