TUDOR RARES OLARIU, OCTAVIAN MAZILU, RODICA MIHAESCU, TITUS UDUDEC, ROMULUS TIMAR, SORIN BOLINTINEANU, CRISTINA PETRESCU, CAMELIA VIDITA GURBAN ASSESSMENT OF GIARDIA LAMBLIA INFECTION PREVALENCE USING LUGOL’S IODINE STAINING TECHNIQUE
Parasitic diseases have a worldwide distribution and represent an important public health problem. The aim of the present study was to evaluate the prevalence of intestinal parasitic infections in a Child Day Care Center from Western Romania using direct wet mount and concentration procedures of stool samples. One hundred sixty-four children aged 2-8 years were investigated. Stool examinations were performed using the iodine staining for the identification of protozoan cysts and the Willis-Hung concentration method for the identification of helminth eggs. Routine laboratory investigations were also conducted to evaluate the eosinophil count in the infected children. Intestinal parasitic infections were diagnosed in 47 cases (28.6%). Giardia lamblia (25%), Entamoeba coli (5.5%), Blastocystis hominis (1.2%), Ascaris lumbricoides (0.6%) and Trichuris trichiura (0.6%) were the only parasites identified. Among the children with parasitic infections we have determined association of two parasites in 7 (14.9%). We have found that in children with giardiasis the eosinophil values were increased (5.54±4.21%) compared to controls (3.69±1.48%) (p<0.01). Our findings suggest that giardiasis may be significantly prevalent in children attending day care centers in Western Romania. Efficient educational programs should be timely implemented in child care centers with the aim of providing appropriate infection control measures.