OANA VIOLA BADULESCU, DAN VINTILA, PAUL DAN SIRBU, BOGDAN MIHNEA CIUNTU THE ROLE OF CHROMOGENIC AND IMMUNOTURBIDIMETRY ASSAYS IN DIAGNOSTIC OF THROMBOTIC STATE IN MYELOPROLIFERATIVE DISORDERS Polycythaemia vera (PV) is a monoclonal myeloproliferative disorder characterized by a haemostatic imbalance resulting in increased risk for thrombotic events. In PV there is an important affection of the vascular endothelium, so we analized in our study three markers of endothelium disfunction- von Willbrand factor (vWF), Protein C (PC) and the inhibitor plasminogen activator type-1. (PAI-1) The study comprised 40 patients and the objective of the study was to investigate the level of the mentioned parameters in two groups of patients-the group of polycytemic patients without cardiovascular diseases (PV) and the group of polycitemic patients with cardiovascular pathology associated (PV+CVD). The levels of the studied parameters were found significantly modified in the PV group (p<0.05); for the PV+CVD group the level of vWF was higher (p<0.001), but the levels of the other parameters, didn’t show a significant statistic difference compared with the first group (p>0.05). In accordance with the presented results, the conclusions of our study was that in PV the risk of thrombotic accidents is related by the increase of the vWF level, as the vascular endothelium plays a key role in this pathology.
Keywords: chromogenic assay; immunoturbidimetry assay, thrombosis; Protein C, enzymatic immunoassay