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https://doi.org/10.37358/Rev.Chim.1949

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Revista de Chimie (Rev. Chim.), Year 2018, Volume 69, Issue 5, 1179-1186

https://doi.org/10.37358/RC.18.5.6284

Teodor Stefanache, Daniela Jitaru, Mihaiela Loredana Dragos, Magda Badescu, Bogdan Mihail Diaconescu, Manuela Ciocoiu, Madalina Mocanu, Codruta Badescu

Tumor Immune Response in the Presence of a Cytotoxic Peptide

Abstract:

We evaluated, by the flow cytometry technique, the viability of two tumor cell lines: colorectal carcinoma (HT-29) and human A549 arcinoma incubated with the cytotoxic peptide LL-37. The results obtained for the two cell lines HT-29 and A549 are significantly different under the action of cathelicidin LL-37. At high concentrations of 20mM, cellular apoptosis was over 30% higher for colorectal adenocarcinoma line compared to that by peptide exposure. Apoptosis was also significant in low-concentration (4uM) catechidine-labeled lung cancer cells for 48 h. Also, optimization of primers was sought to evaluate gene expression for, Bcl2, IL6, IL8. Determination of gene expression for these molecular targets under the action of the cytotoxic peptide was performed in order to evaluate the immune response of tumor cells. For this purpose, the genetic material (RNA) was extracted from cell cultures and reversed in cDNA, which was subsequently amplified by the qRT-PCR technique. Evaluation of tumor cell metabolism was done by determining the gene expression for Bcl2, IL6, IL8 by the action of the cytotoxic peptides used. The cytotoxic effect of cathelicidin LL-37 for the two cell lines HT29 and A549 is supported by the decrease in IL-6 and IL-8 gene expression. The increase in Bcl2 expression for cells exposed to the action of the peptide is explained by the increase in anti-apoptotic protein synthesis that explains the fight of tumor cells for survival and proliferation.
Keywords:
colorectal carcinoma; alveolar carcinoma; cytotoxic peptide

Issue: 2018, Volume 69, Issue 5
Pages: 1179-1186
Publication date: 2018/6/15
https://doi.org/10.37358/RC.18.5.6284
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Creative Commons License
This article is published under the Creative Commons Attribution 4.0 International License
Citation Styles
Cite this article as:
STEFANACHE, T., JITARU, D., DRAGOS, M.L., BADESCU, M., DIACONESCU, B.M., CIOCOIU, M., MOCANU, M., BADESCU, C., Tumor Immune Response in the Presence of a Cytotoxic Peptide, Rev. Chim., 69(5), 2018, 1179-1186.

Vancouver
Stefanache T, Jitaru D, Dragos ML, Badescu M, Diaconescu BM, Ciocoiu M, Mocanu M, Badescu C. Tumor Immune Response in the Presence of a Cytotoxic Peptide. Rev. Chim.[internet]. 2018 May;69(5):1179-1186. Available from: https://doi.org/10.37358/RC.18.5.6284


APA 6th edition
Stefanache, T., Jitaru, D., Dragos, M.L., Badescu, M., Diaconescu, B.M., Ciocoiu, M., Mocanu, M. & Badescu, C. (2018). Tumor Immune Response in the Presence of a Cytotoxic Peptide. Revista de Chimie, 69(5), 1179-1186. https://doi.org/10.37358/RC.18.5.6284


Harvard
Stefanache, T., Jitaru, D., Dragos, M.L., Badescu, M., Diaconescu, B.M., Ciocoiu, M., Mocanu, M., Badescu, C. (2018). 'Tumor Immune Response in the Presence of a Cytotoxic Peptide', Revista de Chimie, 69(5), pp. 1179-1186. https://doi.org/10.37358/RC.18.5.6284


IEEE
T. Stefanache, D. Jitaru, M.L. Dragos, M. Badescu, B.M. Diaconescu, M. Ciocoiu, M. Mocanu, C. Badescu, "Tumor Immune Response in the Presence of a Cytotoxic Peptide". Revista de Chimie, vol. 69, no. 5, pp. 1179-1186, 2018. [online]. https://doi.org/10.37358/RC.18.5.6284


Text
Teodor Stefanache, Daniela Jitaru, Mihaiela Loredana Dragos, Magda Badescu, Bogdan Mihail Diaconescu, Manuela Ciocoiu, Madalina Mocanu, Codruta Badescu,
Tumor Immune Response in the Presence of a Cytotoxic Peptide,
Revista de Chimie,
Volume 69, Issue 5,
2018,
Pages 1179-1186,
ISSN 2668-8212,
https://doi.org/10.37358/RC.18.5.6284.
(https://revistadechimie.ro/Articles.asp?ID=6284)
Keywords: colorectal carcinoma; alveolar carcinoma; cytotoxic peptide


RIS
TY - JOUR
T1 - Tumor Immune Response in the Presence of a Cytotoxic Peptide
A1 - Stefanache, Teodor
A2 - Jitaru, Daniela
A3 - Dragos, Mihaiela Loredana
A4 - Badescu, Magda
A5 - Diaconescu, Bogdan Mihail
A6 - Ciocoiu, Manuela
A7 - Mocanu, Madalina
A8 - Badescu, Codruta
JF - Revista de Chimie
JO - Rev. Chim.
PB - Revista de Chimie SRL
SN - 2668-8212
Y1 - 2018
VL - 69
IS - 5
SP - 1179
EP - 1186
UR - https://doi.org/10.37358/RC.18.5.6284
KW - colorectal carcinoma
KW - alveolar carcinoma
KW - cytotoxic peptide
ER -


BibTex
@article{RevCh2018P1179,
author = {Stefanache Teodor and Jitaru Daniela and Dragos Mihaiela Loredana and Badescu Magda and Diaconescu Bogdan Mihail and Ciocoiu Manuela and Mocanu Madalina and Badescu Codruta},
title = {Tumor Immune Response in the Presence of a Cytotoxic Peptide},
journal = {Revista de Chimie},
volume = {69},
number = {5},
pages = {1179-1186},
year = {2018},
issn = {2668-8212},
doi = {https://doi.org/10.37358/RC.18.5.6284},
url = {https://revistadechimie.ro/Articles.asp?ID=6284}
}
 
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