OANA VIOLA BADULESCU, MAGDA BADESCU, MANUELA CIOCOIU, MADALINA MOCANU FLOW CYTOMETRY WITH INFLORESCENCE-AN ACCESSIBLE METHOD IN THE STUDY OF QUALITATIVE AND QUANTITATIVE MODIFICATIONS OF BLOOD PLATELETS IN IMMUNE THROMBOCYTOPENIC PURPURA The immune thrombocytopenic purpura (ITP) is a polymorphic and hematologic pathology, in terms of both clinical manifestations and etiopathogenic mechanisms, which bring on this disease. Establishing the type of thrombocytopenia, which can either be primary or secondary, peripheral or central, is essential for a further proper therapeutic conduct. The current means of diagnosis include a large variety of hematologic, immunologic and biochemical explorations, from the traditional ones to the latest methods, either genetical or molecular. The purpose of this paper is to emphasize the importance of a classical method of diagnosis, used in the primary triage of patients suffering from thrombocytopenia, highly valuable in the diagnosis process: the analysis of the mean platelet volume (MPV) and of the platelet distribution width (PDW), parameters revealed by the CBC (complete blood count). Evaluating the size of the platelets is a useful tool in the differential diagnosis between immune thrombocytopenic purpura and hereditary macrothrombocytopenia. The platelet distribution width and the mean platelet volume are read simultaneously, in order to differentiate the immune peripheral thrombocytopenia from the central one. The classical, viable and accessible method facilitates the measurement of MPV and PDW with the automatic analyzer, which functions according to the principle of flow cytometry with inflorescence, by using a LASER semiconductor and hydrodynamic focusing.