IONELA DANIELA MORARIU, LILIANA AVASILCAI, MADALINA VIERIU, ALINA DIANA PANAINTE, NELA BIBIRE VALIDATION AND APPLICATION OF AN ANALYSIS METHOD OF FOUR METABOLITES OF NITROFURANS IN HONEY Although nitrofurans are supposed to be absent in foods, they are still used in veterinary medicine for the treatment of infections in animals not bred for consumption. That meant that there are still samples of honey contaminated with residues of nitrofurans because of bees treated with those pharmaceutical substances. Developing accessible methods to detect them is of high interest to food residue monitoring and regulation programs. We propose an immunochemical method as an alternative to detect four toxic metabolites of nitrofurans (1-aminoimidazolidine-2,4-dione, 3-amino-5-morpholinomethyl-2-oxazolidinone, 3-amino-2-oxazolidone and semicarbazide) in honey. The new method has been optimized and validate for the simultaneous determination of the four metabolites of the nitrofurans honey using biochip technology, and it has been used for the quantitative determination of the residues in 16 Romanian honey samples. The evaluated validation parameters included: linearity, sensitivity (IC50 £2.32 mg/kg), specificity and selectivity, precision (intermediate and reproducibility) and accuracy, decision limit (CCa between 0.37 and 1.05 mg/kg), detection capability (CCb between 0.42 and 1.14 mg/kg), and recovery coefficient (64–192%).