IULIA CRISTINA BAGIU, DELIA IOANA HORHAT, SIMONA POPESCU, FLORIN GEORGE HORHAT, CIPRIAN PILUT, RAZVAN NITU, GEORGE DAHMA, AMADEUS DOBRESCU, ELENA AMARICAI, OVIDIU BORUGA, RADU VASILE BAGIU ONE YEAR RETROSPECTIVE STUDY OF OCULAR INFECTION IN A TERTIARY CARE HOSPITAL FROM TIMISOARA
Eye is the most important sensory organ concerned with the perception of vision. Ocular infections are one of the common diseases of the eye. Ocular infections as blepharitis, conjunctivitis, canaliculitis, dacryocystitis, keratitis, scleritis, orbital cellulitis, endophthalmitis, panophthalmitis and other infections which are responsible for increased incidence of morbidity and blindness worldwide, their morbidity vary from self-limiting trivial infection to sight threatening infection. To identify the bacterial profile of ocular infections in patients attending Ophthalmology Department. After clinical diagnosis of ocular infection made by Ophthalmologist, specimens were collected with the help of Ophthalmologist. Samples like eyelid swab, pus from dacryocystitis, corneal scrapings, corneal swab, and tissue specimens from 189 patients attending Ophthalmology Department were analyzed from 01.01.2014 to 01.01.2015. Using predefined inclusion and exclusion criteria, samples were collected according to the standard protocol. Inclusion criteria: 1. Clinically diagnosed cases of ocular infections attending Outpatient Department and Inpatient Department of Ophthalmology, Tertiary Care Center from Timisoara 2. Patients not on antibiotics (systemic or either topical) will be included in the study. 3. Patients not responding to antibiotics. Exclusion criteria: 1. Non-infectious etiology of ocular diseases. The material was examined Gram staining. The specimens were cultured on sheep’s blood agar, Chocolate agar and MacConkey agar, Chapman agar, Sabouraud dextrose agar. Drug susceptibility was tested using disc diffusion method (Kirby Bauer).The most common bacterial pathogen isolated were Staphylococcus aureus (29.7%) followed by Staphylococcus epidermidis (22.1%), other organisms isolated are Streptococcus pneumonia (17.9%), Klebsiella spp. (6.3%), Escherichia coli (4.7%), Pseudomonas aeruginosa (3.4%). Among the opportunistic pathogens, Staphylococcus epidermidis, (22.1%) were the most common isolate followed by Staphylococcus saprophyticus (0.45%). Bacterial isolates were highly susceptible to Vancomycin (100%), Gentamicin (92.1%) among Gram positive organisms. The Gram negative organisms are highly susceptible to Tobramycin (95.4%) and Imipenem (87.9%). The study suggests that Staphylococcus aureus and Staphylococcus epidermidis are the most common etiological agents of Ocular infections. Most of the strains were sensitive to Vancomycin and Tobramycin. Persistent efforts should be put for continuous surveillance and epidemiological characterization which are imperative to treat and prevent morbidity and blindness of population at risk.