In vitro Response of Human Osteoblasts Cultured on Strontium Substituted Hydroxyapatites

Rapuntean, Sorin; Frangopol, Petre T.; Hodisan, Ioana; Tomoaia, Gheorghe; Oltean-Dan, Daniel; Mocanu, Aurora; Prejmerean, Cristina; Soritau, Olga; Racz, Levente Zsolt; Tomoaia-Cotisel, Maria

Sorin Rapuntean, Petre T. Frangopol, Ioana Hodisan, Gheorghe Tomoaia, Daniel Oltean-Dan, Aurora Mocanu, Cristina Prejmerean, Olga Soritau, Levente Zsolt Racz, Maria Tomoaia-Cotisel

In vitro Response of Human Osteoblasts Cultured on Strontium Substituted Hydroxyapatites

Abstract:

The goal of this study was to analyze the response of osteoblasts cultured on strontium substituted hydroxyapatites (HAP-Sr) of well-defined high crystallinity deposited as thin films on glass plates. Up to now, this aspect has not been carefully investigated in the context of bio-ceramics. In this study, we present the osteoblasts activity on synthesized HAP-Sr for different amounts of strontium substitution for calcium within the hydroxyapatite (Ca10(PO4)6(OH)2, HAP) lattice, namely HAP-5%Sr, HAP-10%Sr, HAP-15%Sr and HAP-59.2%Sr (Sr-HAP, of formula Sr10(PO4)6(OH)2), in comparison with stoichiometric pure HAP, chosen as control. Each bio-ceramic was deposited as thin multilayers self-assembled substrate (scaffold) and chemically bonded to the surface of glass plates. These coatings revealed by AFM and SEM imaging a granular texture formed from bio-ceramic nanoparticles. They possessed a high degree of crystallinity, i.e. 68% to 86%, depending on the Sr amount within the HAP lattice, as judged by XRD. Osteoblasts were cultured up to 21days and displayed enhanced adhesion and proliferation particularly evidenced on relatively high strontium contents (especially 5 and 10 weight %, determined by SEM-EDX), where the alkaline phosphatase activity and type I collagen were strongly evidenced. These bio-ceramics showed a high in vitro biocompatibility stimulating the activity of osteoblasts in the process of bone formation. These nano biomaterials can have applications in orthopedic and dental surgery improving the osteointegration as coatings of bone implants as well as for bone repair and regeneration.

Keywords:
ceramics; strontium substituted hydroxyapatite; osteoblasts; alkaline phosphatase activity; collagen; cellular viability

Issue: 2018, Volume 69, Issue 12

Pages: 3537-3444

Publication date: 2019/1/15

https://doi.org/10.37358/RC.18.12.6787

Download Pdf Article

This article is published under the Creative Commons Attribution 4.0 International License

Citation Styles

Cite this article as:
RAPUNTEAN, S., FRANGOPOL, P.T., HODISAN, I., TOMOAIA, G., OLTEAN-DAN, D., MOCANU, A., PREJMEREAN, C., SORITAU, O., RACZ, L.Z., TOMOAIA-COTISEL, M., In vitro Response of Human Osteoblasts Cultured on Strontium Substituted Hydroxyapatites, Rev. Chim., 69(12), 2018, 3537-3444.

Vancouver
Rapuntean S, Frangopol PT, Hodisan I, Tomoaia G, Oltean-Dan D, Mocanu A, Prejmerean C, Soritau O, Racz LZ, Tomoaia-Cotisel M. In vitro Response of Human Osteoblasts Cultured on Strontium Substituted Hydroxyapatites. Rev. Chim.[internet]. 2018 Dec;69(12):3537-3444. Available from: https://doi.org/10.37358/RC.18.12.6787

APA 6th edition
Rapuntean, S., Frangopol, P.T., Hodisan, I., Tomoaia, G., Oltean-Dan, D., Mocanu, A.,... Tomoaia-Cotisel, M. (2018). In vitro Response of Human Osteoblasts Cultured on Strontium Substituted Hydroxyapatites. Revista de Chimie, 69(12), 3537-3444. https://doi.org/10.37358/RC.18.12.6787

Harvard
Rapuntean, S., Frangopol, P.T., Hodisan, I., Tomoaia, G., Oltean-Dan, D., Mocanu, A., Prejmerean, C., Soritau, O., Racz, L.Z., Tomoaia-Cotisel, M. (2018). 'In vitro Response of Human Osteoblasts Cultured on Strontium Substituted Hydroxyapatites', Revista de Chimie, 69(12), pp. 3537-3444. https://doi.org/10.37358/RC.18.12.6787

IEEE
S. Rapuntean, P.T. Frangopol, I. Hodisan, G. Tomoaia, D. Oltean-Dan, A. Mocanu, C. Prejmerean, O. Soritau, L.Z. Racz, M. Tomoaia-Cotisel, "In vitro Response of Human Osteoblasts Cultured on Strontium Substituted Hydroxyapatites". Revista de Chimie, vol. 69, no. 12, pp. 3537-3444, 2018. [online]. https://doi.org/10.37358/RC.18.12.6787

Text
Sorin Rapuntean, Petre T. Frangopol, Ioana Hodisan, Gheorghe Tomoaia, Daniel Oltean-Dan, Aurora Mocanu, Cristina Prejmerean, Olga Soritau, Levente Zsolt Racz, Maria Tomoaia-Cotisel,
In vitro Response of Human Osteoblasts Cultured on Strontium Substituted Hydroxyapatites,
Revista de Chimie,
Volume 69, Issue 12,
2018,
Pages 3537-3444,
ISSN 2668-8212,
https://doi.org/10.37358/RC.18.12.6787.
(https://revistadechimie.ro/Articles.asp?ID=6787)
Keywords: ceramics; strontium substituted hydroxyapatite; osteoblasts; alkaline phosphatase activity; collagen; cellular viability

RIS
TY - JOUR
T1 - In vitro Response of Human Osteoblasts Cultured on Strontium Substituted Hydroxyapatites
A1 - Rapuntean, Sorin
A2 - Frangopol, Petre T.
A3 - Hodisan, Ioana
A4 - Tomoaia, Gheorghe
A5 - Oltean-Dan, Daniel
A6 - Mocanu, Aurora
A7 - Prejmerean, Cristina
A8 - Soritau, Olga
A9 - Racz, Levente Zsolt
A10 - Tomoaia-Cotisel, Maria
JF - Revista de Chimie
JO - Rev. Chim.
PB - Revista de Chimie SRL
SN - 2668-8212
Y1 - 2018
VL - 69
IS - 12
SP - 3537
EP - 3444
UR - https://doi.org/10.37358/RC.18.12.6787
KW - ceramics
KW - strontium substituted hydroxyapatite
KW - osteoblasts
KW - alkaline phosphatase activity
KW - collagen
KW - cellular viability
ER -

BibTex
@article{RevCh2018P3537,
author = {Rapuntean Sorin and Frangopol Petre T. and Hodisan Ioana and Tomoaia Gheorghe and Oltean-Dan Daniel and Mocanu Aurora and Prejmerean Cristina and Soritau Olga and Racz Levente Zsolt and Tomoaia-Cotisel Maria},
title = {In vitro Response of Human Osteoblasts Cultured on Strontium Substituted Hydroxyapatites},
journal = {Revista de Chimie},
volume = {69},
number = {12},
pages = {3537-3444},
year = {2018},
issn = {2668-8212},
doi = {https://doi.org/10.37358/RC.18.12.6787},
url = {https://revistadechimie.ro/Articles.asp?ID=6787}
}

<<<< back

PUBLISHING SECTION

Author Guidelines

* Paper Drafting

* Paper Template

* Ethical Statement

* Paper Submission

Ghidul Autorului (ro)

Open Access & Archiving Policy

Search Authors

LATEST NEWS
	NUMAR OMAGIAL PETRU PONI.. detalii
	Revista de Chimie - 70 de ani de aparitie neintrerupta.. detalii
	English-Romanian Dictionary for Mechanical Engineering (download pdf)