ALEXANDRA TOTAN, DANIELA GABRIELA BALAN, DANIELA MIRICESCU, RADU RADULESCU, IULIA IOANA STANESCU, BOGDANA VIRGOLICI, MARIA MOHORA, MARIA GREABU NONALCOHOLIC FATTY LIVER DISEASE AND VITAMIN E - A PROMISING RELATIONSHIP? Oxidative stress (OS) plays an important role in NAFLD molecular mechanism. Nanoencapsulation represents a novel strategy to enhance therapeutic potential of conventional drugs. Our study analyses the encapsulated vitamin E effect on lipid metabolism and oxidative stress biomarkers in NAFLD rats. Animals were divided into 3 groups : G1 - the normal diet group; G2- the high caloric diet group ; G3 - high-caloric diet group receiving PLGA-vit E, 50 mg / kg. Serum advanced human oxidative protein (AOPP), total antioxidant capacity (TAC) and vitamin E were analysed using ELISA technique. Our results showed significant increase of G2 GPT, ALP, GGT, TG, glucose, TC and AOPP, versus G1 ( P < 0.05) and a significant decrease of G2 serum TAC and vitamin E versus G1 results ( p = 0.01 and 0.01). Vitamin E nanoparticles (G3) caused a significant increase of TAC and significant decrease of serum AOPP, versus G2 (p < 0.01). Results showed a significant reduction of GPT, GGT, ALP, TG and total cholesterol ( p <0.05) in G3 versus G2. PLGA nanoparticles should be considered an attractive and promising alternative to improve the bioavailability and biological activity of vitaminE.
Keywords: nanoparticles, vitamin E, oxidative stress, liver disease