DELIA MIRA BERCEANU VADUVA, DANA EMILIA VELIMIROVICI, LIVIA STANGA, MARCEL MIHAI BERCEANU VADUVA, SMARANDA ARGHIRESCU1, ADRIAN STANCU3, HORATIU PETRESCU, MATILDA RADULESCU SIGNIFICANCE OF SPECIFIC IMMUNOGLOBULINS DETERMINED BY THE CHEMILUMINESCENCE REACTION IN INTRAUTERINE MATERNAL-FETAL INFECTIONS OF TORCH SYNDROME Because the TORCH Syndrome groups infectious agents which cross the placenta during pregnancy and due to that cause the majority of malformative fetopaties, our porpose was to determin specific IgG and IgM antibodies for Cytomegalovirus, Rubella virus and Toxoplasma gondii. We studied 95 serum samples from 93 pregnant females and 2 cilds, being analyzed by a private laboratory in Timisoara between july 2015 – june 2016. Specific antibodies IgG and IgM were found for Rubella virus in 75 of the samples, for Cytomegalovirus in 82 and for toxoplasmosis 92. In this process of determination we used Access 2 (Beckman Coulter) and Immulite 1000 (Siemens) analysers, which use the chemiluminescence reaction. For 72 cases (77.42%) specific antibodies for all tests of the TORCH complex were performed. From all the patients 11 chosed to determine antibodies just for one of the infections of the TORCH Syndrome and 10 patients asked for determining of specific antibodies for two infections of the TORCH Syndrome. 53 (64.63%) of the 82 patients tested for anti-CMV IgG specific antibodies had positive result, which signifies former presence of CMV. 64 (85.33%) of the 75 patients tested for anti-rubella specific antibodies had an IgG positive result. Interpreting of results took in counter the vaccinal status. 6 patients (8%) did not contact the Rubella virus and did were not vaccinated. The level of specific IgG anti-Toxoplasma gondii antibodies was positive for 2 (2.17%) patients of 92. IgM specific antibody test was negative. In pregnant women TORCH Syndrome diagnosis is a must, especially if the medical abortion is the correct decision. Identifyeing infectious agents implicated in TORCH Syndrome can lead to an important decrease of maternal and fetal mortality and morbidity.
Keywords: TORCH Syndrome, Cytomegalovirus, Rubella virus, Toxoplasma gondii, IgG and IgM antibodies/immunoglobulines, the chemiluminescence reaction