GABRIELA STANCIU, SIMONA LUPSOR, ANETA TOMESCU, RODICA SIRBU EVALUATION OF ANTIOXIDANT CAPACITIES AND PHENOLS COMPOSITION OF WILD AND CULTIVATED BERRIES The study presents original results concerning analytical characterization of alcoholic extracts of four wild berries (blueberries, blackberries, red currants and raspberries) and two cultivated berries (black cherries and strawberries). Total and some individual phenols concentrations were determined using molecular absorption spectrometry (modified Folin Ciocalteu method) and HPLC-DAD technique. The measurement of lipid-soluble antioxidant capacity (ACL) was done by photochemiluminescence method using trolox as standard.The highest total phenolic content was registered in blueberries (543.5 mg/100g f.w.), while the lowest was found in raspberries (344.5 mg/100g f.w.).HPLC – DAD analysis indicated the presence of six individual polyphenolic compounds in different concentrations: gallic acid (62.664 - 178.821 mg/100 g f.w); chlorogenic acid (30.152 - 243.923 mg/100g f.w.); 3-O-methyl-gallic acid (2.035 - 4.907 mg/100 g f.w.); caffeic acid (0.401 - 5.664 mg/100 g f.w.); p-coumaric acid (4.252 - 10.806 mg/100 g f.w.) and cinnamic acid (0.665-0.958 mg/100 g f.w.). ACL results showed values between 54.00 and 1152 µmols trolox/100 g f.w.
Keywords: berries, total phenols, antioxidant capacity of soluble lipids, HPLC-DAD chromatography