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REVISTA DE CHIMIE
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https://doi.org/10.37358/Rev.Chim.1949

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Revista de Chimie (Rev. Chim.), Year 2019, Volume 70, Issue 2, 532-535

https://doi.org/10.37358/RC.19.2.6950

Irina Cezara Vacarean Trandafir, Iuliu Cristian Ivanov, Loredana Mihaiela Dragos, Amalia Andrea Titieanu, Dumitru Cojocaru

Detection of IG and TCR Monoclonality as a Molecular Marker in Acute Hematopathies

Abstract:

The aim of this study was to use clonal gene rearrangements assays to discriminate between oligo-clonal or polyclonal reactive processes in cases that presented difficulty in diagnosis of B or T-acute lymphoblastic leukemia (ALL) due to abnormal phenotypes detected by flow-cytometry. Monoclonal B /T cells were detected by rearrangements of immunoglobulin (Ig) and T-cell receptors (TCR) in 10 patients. After DNA extraction from peripheral blood, multiplex PCR was used to amplify the Ig/TCR gene rearrangements followed by capillary electrophoresis, according to BIOMED 2 conditions. The evaluation of the results was carried out in the clinical and interdisciplinary (histomorphological, molecular and flow-cytometric) context. The presence of clonal rearrangements may indicate the existence of two neoplastic processes or a single clone with two gene rearrangements - one productive and one non-productive on the two alleles, considering the use of genomic DNA. By fragment analysis we identified B/T cells gene recombinations using multiplex PCR with primer sets that target each type of gene family. Using these molecular markers we can discriminate between monoclonal/polyclonal cells and follow a clonal marker throughout the disease evolution.
Keywords:
ALL; Ig/TCR; gene rearrangement; BIOMED2; monoclonal

Issue: 2019, Volume 70, Issue 2
Pages: 532-535
Publication date: 2019/3/15
https://doi.org/10.37358/RC.19.2.6950
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Creative Commons License
This article is published under the Creative Commons Attribution 4.0 International License
Citation Styles
Cite this article as:
TRANDAFIR, I.C.V., IVANOV, I.C., DRAGOS, L.M., TITIEANU, A.A., COJOCARU, D., Detection of IG and TCR Monoclonality as a Molecular Marker in Acute Hematopathies, Rev. Chim., 70(2), 2019, 532-535.

Vancouver
Trandafir ICV, Ivanov IC, Dragos LM, Titieanu AA, Cojocaru D. Detection of IG and TCR Monoclonality as a Molecular Marker in Acute Hematopathies. Rev. Chim.[internet]. 2019 Feb;70(2):532-535. Available from: https://doi.org/10.37358/RC.19.2.6950


APA 6th edition
Trandafir, I.C.V., Ivanov, I.C., Dragos, L.M., Titieanu, A.A. & Cojocaru, D. (2019). Detection of IG and TCR Monoclonality as a Molecular Marker in Acute Hematopathies. Revista de Chimie, 70(2), 532-535. https://doi.org/10.37358/RC.19.2.6950


Harvard
Trandafir, I.C.V., Ivanov, I.C., Dragos, L.M., Titieanu, A.A., Cojocaru, D. (2019). 'Detection of IG and TCR Monoclonality as a Molecular Marker in Acute Hematopathies', Revista de Chimie, 70(2), pp. 532-535. https://doi.org/10.37358/RC.19.2.6950


IEEE
I.C.V. Trandafir, I.C. Ivanov, L.M. Dragos, A.A. Titieanu, D. Cojocaru, "Detection of IG and TCR Monoclonality as a Molecular Marker in Acute Hematopathies". Revista de Chimie, vol. 70, no. 2, pp. 532-535, 2019. [online]. https://doi.org/10.37358/RC.19.2.6950


Text
Irina Cezara Vacarean Trandafir, Iuliu Cristian Ivanov, Loredana Mihaiela Dragos, Amalia Andrea Titieanu, Dumitru Cojocaru,
Detection of IG and TCR Monoclonality as a Molecular Marker in Acute Hematopathies,
Revista de Chimie,
Volume 70, Issue 2,
2019,
Pages 532-535,
ISSN 2668-8212,
https://doi.org/10.37358/RC.19.2.6950.
(https://revistadechimie.ro/Articles.asp?ID=6950)
Keywords: ALL; Ig/TCR; gene rearrangement; BIOMED2; monoclonal


RIS
TY - JOUR
T1 - Detection of IG and TCR Monoclonality as a Molecular Marker in Acute Hematopathies
A1 - Trandafir, Irina Cezara Vacarean
A2 - Ivanov, Iuliu Cristian
A3 - Dragos, Loredana Mihaiela
A4 - Titieanu, Amalia Andrea
A5 - Cojocaru, Dumitru
JF - Revista de Chimie
JO - Rev. Chim.
PB - Revista de Chimie SRL
SN - 2668-8212
Y1 - 2019
VL - 70
IS - 2
SP - 532
EP - 535
UR - https://doi.org/10.37358/RC.19.2.6950
KW - ALL
KW - Ig/TCR
KW - gene rearrangement
KW - BIOMED2
KW - monoclonal
ER -


BibTex
@article{RevCh2019P532,
author = {Trandafir Irina Cezara Vacarean and Ivanov Iuliu Cristian and Dragos Loredana Mihaiela and Titieanu Amalia Andrea and Cojocaru Dumitru},
title = {Detection of IG and TCR Monoclonality as a Molecular Marker in Acute Hematopathies},
journal = {Revista de Chimie},
volume = {70},
number = {2},
pages = {532-535},
year = {2019},
issn = {2668-8212},
doi = {https://doi.org/10.37358/RC.19.2.6950},
url = {https://revistadechimie.ro/Articles.asp?ID=6950}
}
 
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