MELANIA BANU, MONICA SIMION, MIHAELA KUSKO, ILEANA C. FARCASANU ENHANCING THE MICROARRAY SIGNAL DETECTION OF SINGLE NUCLEOTIDE POLYMORPHISMS (SNPS) BY USING HOMEMADE IMMOBILISATION BUFFERS Our research is focused on improving the detection capabilities of single nucleotide polymorphisms (SNPs) with pathological significance within breast cancer 1, early onset gene (BRCA1). We employed the BRCA1 canonical probe sequence containing the common C allele and probes including the mismatches caused by C replacement with any of the other nucleotides (A, G or T). Two immobilization buffers with distinct pH and salt concentration were involved in the experimental and statistical analyses in order to assess the detection specificity and stability over time of the tethered probes in oligonucleotide microarrays. A high spreading of data points (standard deviation 0.16) was acquired from the fluorescent hybridization signal of the DNA probes diluted in Phosphate Buffered Saline (PBS), while the Disodium Phosphate (Na2HPO4) buffer was recommended by a standard deviation of 0.08 for further investigations. The high specificity of mismatch detection of DNA probes diluted in Na2HPO4 buffer was established by Student’s t test, revealing the statistical reliability of the DNA-printed slides after 1, 4, 8 and 21 days.