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https://doi.org/10.37358/Rev.Chim.1949

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Revista de Chimie (Rev. Chim.), Year 2019, Volume 70, Issue 2, 730-735

https://doi.org/10.37358/RC.19.2.6995

Melania Banu, Monica Simion, Mihaela Kusko, Ileana C. Farcasanu

Enhancing the Microarray Signal Detection of Single Nucleotide Polymorphisms (SNPs) by Using Homemade Immobilisation Buffers

Abstract:

Our research is focused on improving the detection capabilities of single nucleotide polymorphisms (SNPs) with pathological significance within breast cancer 1, early onset gene (BRCA1). We employed the BRCA1 canonical probe sequence containing the common C allele and probes including the mismatches caused by C replacement with any of the other nucleotides (A, G or T). Two immobilization buffers with distinct pH and salt concentration were involved in the experimental and statistical analyses in order to assess the detection specificity and stability over time of the tethered probes in oligonucleotide microarrays. A high spreading of data points (standard deviation 0.16) was acquired from the fluorescent hybridization signal of the DNA probes diluted in Phosphate Buffered Saline (PBS), while the Disodium Phosphate (Na2HPO4) buffer was recommended by a standard deviation of 0.08 for further investigations. The high specificity of mismatch detection of DNA probes diluted in Na2HPO4 buffer was established by Student’s t test, revealing the statistical reliability of the DNA-printed slides after 1, 4, 8 and 21 days.
Keywords:
Microarray technology; immobilisation buffers; probe stability; SNP; BRCA1

Issue: 2019, Volume 70, Issue 2
Pages: 730-735
Publication date: 2019/3/15
https://doi.org/10.37358/RC.19.2.6995
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Creative Commons License
This article is published under the Creative Commons Attribution 4.0 International License
Citation Styles
Cite this article as:
BANU, M., SIMION, M., KUSKO, M., FARCASANU, I.C., Enhancing the Microarray Signal Detection of Single Nucleotide Polymorphisms (SNPs) by Using Homemade Immobilisation Buffers, Rev. Chim., 70(2), 2019, 730-735.

Vancouver
Banu M, Simion M, Kusko M, Farcasanu IC. Enhancing the Microarray Signal Detection of Single Nucleotide Polymorphisms (SNPs) by Using Homemade Immobilisation Buffers. Rev. Chim.[internet]. 2019 Feb;70(2):730-735. Available from: https://doi.org/10.37358/RC.19.2.6995


APA 6th edition
Banu, M., Simion, M., Kusko, M. & Farcasanu, I.C. (2019). Enhancing the Microarray Signal Detection of Single Nucleotide Polymorphisms (SNPs) by Using Homemade Immobilisation Buffers. Revista de Chimie, 70(2), 730-735. https://doi.org/10.37358/RC.19.2.6995


Harvard
Banu, M., Simion, M., Kusko, M., Farcasanu, I.C. (2019). 'Enhancing the Microarray Signal Detection of Single Nucleotide Polymorphisms (SNPs) by Using Homemade Immobilisation Buffers', Revista de Chimie, 70(2), pp. 730-735. https://doi.org/10.37358/RC.19.2.6995


IEEE
M. Banu, M. Simion, M. Kusko, I.C. Farcasanu, "Enhancing the Microarray Signal Detection of Single Nucleotide Polymorphisms (SNPs) by Using Homemade Immobilisation Buffers". Revista de Chimie, vol. 70, no. 2, pp. 730-735, 2019. [online]. https://doi.org/10.37358/RC.19.2.6995


Text
Melania Banu, Monica Simion, Mihaela Kusko, Ileana C. Farcasanu,
Enhancing the Microarray Signal Detection of Single Nucleotide Polymorphisms (SNPs) by Using Homemade Immobilisation Buffers,
Revista de Chimie,
Volume 70, Issue 2,
2019,
Pages 730-735,
ISSN 2668-8212,
https://doi.org/10.37358/RC.19.2.6995.
(https://revistadechimie.ro/Articles.asp?ID=6995)
Keywords: Microarray technology; immobilisation buffers; probe stability; SNP; BRCA1


RIS
TY - JOUR
T1 - Enhancing the Microarray Signal Detection of Single Nucleotide Polymorphisms (SNPs) by Using Homemade Immobilisation Buffers
A1 - Banu, Melania
A2 - Simion, Monica
A3 - Kusko, Mihaela
A4 - Farcasanu, Ileana C.
JF - Revista de Chimie
JO - Rev. Chim.
PB - Revista de Chimie SRL
SN - 2668-8212
Y1 - 2019
VL - 70
IS - 2
SP - 730
EP - 735
UR - https://doi.org/10.37358/RC.19.2.6995
KW - Microarray technology
KW - immobilisation buffers
KW - probe stability
KW - SNP
KW - BRCA1
ER -


BibTex
@article{RevCh2019P730,
author = {Banu Melania and Simion Monica and Kusko Mihaela and Farcasanu Ileana C.},
title = {Enhancing the Microarray Signal Detection of Single Nucleotide Polymorphisms (SNPs) by Using Homemade Immobilisation Buffers},
journal = {Revista de Chimie},
volume = {70},
number = {2},
pages = {730-735},
year = {2019},
issn = {2668-8212},
doi = {https://doi.org/10.37358/RC.19.2.6995},
url = {https://revistadechimie.ro/Articles.asp?ID=6995}
}
 
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