Lectin from Pisum fulvum Seeds as in vitro Anticancer and Apoptotic Gene Regulator

Yassin, Abdelrahman Mohamed; El-Deeb, Nehal Mohamed; Elsaid, Fahmy Gad; Shati, Ali Abdullah; Cioca, Gabriela; Tit, Delia Mirela; Bungau, Simona; Popa, Amorin; Hafez, Elsayed Elsayed

Abdelrahman Mohamed Yassin, Nehal Mohamed El-Deeb, Fahmy Gad Elsaid, Ali Abdullah Shati, Gabriela Cioca, Delia Mirela Tit, Simona Bungau, Amorin Popa, Elsayed Elsayed Hafez

Lectin from Pisum fulvum Seeds as in vitro Anticancer and Apoptotic Gene Regulator

Abstract:

The lectins are non-immune origin carbohydrate-binding proteins. Plant’s lectins are distributed in many species of medicinal plants, family Fabaceae. In this study the safety usage pattern of wild Pisum fulvum lectin was evaluated on different mammalian noncancerous cell types and the anticancer activity was examined on different cancer human cell lines: colorectal adenocarcinoma (Caco-2), hepatocellular carcinoma (HepG2), breast cancer cells (MCF7) and laryngeal carcinoma (Hep-2 cells). Moreover, both morphological and molecular evidence of apoptosis have been detected using both acridine orange/ethidium bromide (AO/EB) stain and RT-qPCR. The results revealed that IC50 of the wild lectin on the noncancerous cells ranged from 19.7 to 2.4 µg protein/mL. In addition, lectin was more potent against HepG2 cells than the other used cells, with inhibition percentages ranged from 68.45 to 90.98 and with cancer cell selectivity index ranged 3.5 to 28.14. The treatment showed 67.6% inhibition of BrdU incorporation in the proliferated hepatocellular carcinoma cells. Furthermore, HepG2-lectin treated cells showed obvious nuclear condensation after 48 h of treatment with ability to down-regulate the expression of BCL2 and BAX and to up-regulate the expression of Ikab gene. The results obtained in this research work clearly indicated the Pisum fulvum lectin could be a promising potential anticancer agent.

Keywords:
lectin; Pisum fulvum; Fabaceae; cancer cell lines; antioxidant; anticancer

Issue: 2019, Volume 70, Issue 4

Pages: 1490-1495

Publication date: 2019/5/15

https://doi.org/10.37358/RC.19.4.7156

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This article is published under the Creative Commons Attribution 4.0 International License

Citation Styles

Cite this article as:
YASSIN, A.M., EL-DEEB, N.M., ELSAID, F.G., SHATI, A.A., CIOCA, G., TIT, D.M., BUNGAU, S., POPA, A., HAFEZ, E.E., Lectin from Pisum fulvum Seeds as in vitro Anticancer and Apoptotic Gene Regulator, Rev. Chim., 70(4), 2019, 1490-1495.

Vancouver
Yassin AM, El-Deeb NM, Elsaid FG, Shati AA, Cioca G, Tit DM, Bungau S, Popa A, Hafez EE. Lectin from Pisum fulvum Seeds as in vitro Anticancer and Apoptotic Gene Regulator. Rev. Chim.[internet]. 2019 Apr;70(4):1490-1495. Available from: https://doi.org/10.37358/RC.19.4.7156

APA 6th edition
Yassin, A.M., El-Deeb, N.M., Elsaid, F.G., Shati, A.A., Cioca, G., Tit, D.M.,... Hafez, E.E. (2019). Lectin from Pisum fulvum Seeds as in vitro Anticancer and Apoptotic Gene Regulator. Revista de Chimie, 70(4), 1490-1495. https://doi.org/10.37358/RC.19.4.7156

Harvard
Yassin, A.M., El-Deeb, N.M., Elsaid, F.G., Shati, A.A., Cioca, G., Tit, D.M., Bungau, S., Popa, A., Hafez, E.E. (2019). 'Lectin from Pisum fulvum Seeds as in vitro Anticancer and Apoptotic Gene Regulator', Revista de Chimie, 70(4), pp. 1490-1495. https://doi.org/10.37358/RC.19.4.7156

IEEE
A.M. Yassin, N.M. El-Deeb, F.G. Elsaid, A.A. Shati, G. Cioca, D.M. Tit, S. Bungau, A. Popa, E.E. Hafez, "Lectin from Pisum fulvum Seeds as in vitro Anticancer and Apoptotic Gene Regulator". Revista de Chimie, vol. 70, no. 4, pp. 1490-1495, 2019. [online]. https://doi.org/10.37358/RC.19.4.7156

Text
Abdelrahman Mohamed Yassin, Nehal Mohamed El-Deeb, Fahmy Gad Elsaid, Ali Abdullah Shati, Gabriela Cioca, Delia Mirela Tit, Simona Bungau, Amorin Popa, Elsayed Elsayed Hafez,
Lectin from Pisum fulvum Seeds as in vitro Anticancer and Apoptotic Gene Regulator,
Revista de Chimie,
Volume 70, Issue 4,
2019,
Pages 1490-1495,
ISSN 2668-8212,
https://doi.org/10.37358/RC.19.4.7156.
(https://revistadechimie.ro/Articles.asp?ID=7156)
Keywords: lectin; Pisum fulvum; Fabaceae; cancer cell lines; antioxidant; anticancer

RIS
TY - JOUR
T1 - Lectin from Pisum fulvum Seeds as in vitro Anticancer and Apoptotic Gene Regulator
A1 - Yassin, Abdelrahman Mohamed
A2 - El-Deeb, Nehal Mohamed
A3 - Elsaid, Fahmy Gad
A4 - Shati, Ali Abdullah
A5 - Cioca, Gabriela
A6 - Tit, Delia Mirela
A7 - Bungau, Simona
A8 - Popa, Amorin
A9 - Hafez, Elsayed Elsayed
JF - Revista de Chimie
JO - Rev. Chim.
PB - Revista de Chimie SRL
SN - 2668-8212
Y1 - 2019
VL - 70
IS - 4
SP - 1490
EP - 1495
UR - https://doi.org/10.37358/RC.19.4.7156
KW - lectin
KW - Pisum fulvum
KW - Fabaceae
KW - cancer cell lines
KW - antioxidant
KW - anticancer
ER -

BibTex
@article{RevCh2019P1490,
author = {Yassin Abdelrahman Mohamed and El-Deeb Nehal Mohamed and Elsaid Fahmy Gad and Shati Ali Abdullah and Cioca Gabriela and Tit Delia Mirela and Bungau Simona and Popa Amorin and Hafez Elsayed Elsayed},
title = {Lectin from Pisum fulvum Seeds as in vitro Anticancer and Apoptotic Gene Regulator},
journal = {Revista de Chimie},
volume = {70},
number = {4},
pages = {1490-1495},
year = {2019},
issn = {2668-8212},
doi = {https://doi.org/10.37358/RC.19.4.7156},
url = {https://revistadechimie.ro/Articles.asp?ID=7156}
}

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