ROXANA LIANA STAN, BOGDAN SEVASTRE, ADRIANA CORINA HANGAN, SANDA BOTA, DANIELA HANGANU, CORINA MARIA LUCIA IONESCU, CRISTIAN POPOVICI, ALEXANDRA CRISTINA SEVASTRE-BERGHIAN6, ALEXANDRA DREANCA, LAURA GRATIELA VICAS CHEMICAL CHARACTERIZATION OF ARTEMISIA ANNUA L. EXTRACT ASSESSMENT OF ANTIOXIDANT ACTIVITY IN VITRO AND IN VIVO TOXICITY STUDIES The purpose of the study was to demonstrate in vitro antioxidant activity and in vivo toxicity of the Artemisia Annua L. extract. The plant was harvested from Bihor area (Cri’ul Repede and Negru river valleys), Romania. Preparation of the plant product and of the lyophilizated extract was carried out in accordance with the Romanian Pharmacopeia Xth Edition. Lyophilized extract was evaluated in terms of polyphenol content using HPLC method. Antioxidant activity was highlighted using the DPPH, ABTS and FRAP methods. Hepatic, renal and haematological toxicity studies have been performed on laboratory mice. For this purpose blood and organs were collected. Biochemical and haematological parameters were determined on the blood samples and histopathological examination was performed on organs. In vitro antioxidant effect of Artemisia Annua L extract and its lack of in vivo toxicity were demonstrated. It is desirable to obtain a new phytoproduct harvested from spontaneous flora of Romania with antioxidant / antitumoral properties and which is devoid of toxicity.