Immunohistochemical Study of Cell Proliferation in Hepatocellular Carcinoma

Goldis, Adrian; Goldis, Ramona; Cornianu, Marioara; Basa, Norina; Lazar, Daniela; Dobrescu, Amadeus; Lazar, Fulger

Adrian Goldis, Ramona Goldis, Marioara Cornianu, Norina Basa, Daniela Lazar, Amadeus Dobrescu, Fulger Lazar

Immunohistochemical Study of Cell Proliferation in Hepatocellular Carcinoma

Abstract:

Hepatocellular carcinoma (HCC), the most common primary liver cancer, with a poor prognosis, is the fifth most common cancer in men and the seventh in women. The study was made on 32 surgically removed liver carcinomas. In order to compare results, we included a group of non-tumor lesions obtained by liver biopsy. Assessment of the proliferative activity of the studied liver lesions was made using immunohistochemical stains with the monoclonal Ki-67 antibody, clone MIB-1 ready-to-use (DAKO Cytomation CA, USA), in the LSAB-HRP work system. To appreciate the proliferation index of Ki-67 (PI Ki-67), we used the semi-automated method of counting the nuclei on digital images. The statistical analysis was performed using SPSS software, Version 20.0 (IBM SPSS Statistics) and Microsoft Office Excel 2007. Mean value of Ki-67 index was 0.4 ± 0.2 in normal liver, 3.52 ± 0.2 in non-tumor liver lesions and 13.4±7.7 in HCC (p [ 0.001). In chronic hepatitis, PI Ki-67 varied between 2.5 and 5.8 %, with a mean value of 5.2% in portal chronic hepatitis and 5.5% in active chronic hepatitis with cirrhotic evolution. In HCC, the values of Ki-67 index were between 0.7% and 52%, with a mean of PI Ki-67 of 13.43±7.7. 66.6% of HCC associated with hepatitis B virus infection and those developped from a cirrhotic lesion had a high Ki-67 score (p = 0.1). The results we obtained showed: a low Ki-67 score in patients with well-differentiated HCC (G1) (p[0.001), with or without capsule infiltration (p = 0.003); high PI Ki-67 in 33.33% of HCC detected in the right hepatic lobe and those extended bilaterally at the moment of diagnosis (p = 0.142) and a significant relationship between high Ki-67 score and vascular invasion (p [ 0.001), the presence of intrahepatic metastasis being correlated with a high proliferative rate (p [ 0.001). Differences between the proliferation rate of HCC and non-tumor liver lesions (p [ 0.001) show that the uncontrolled division of tumor cells can play an important role in the developpment of HCC.

Keywords:
hepatocellular carcinoma; proliferation index of Ki-67; immunohistochemistry

Issue: 2019, Volume 70, Issue 6

Pages: 2198-2203

Publication date: 2019/7/15

https://doi.org/10.37358/RC.19.6.7304

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This article is published under the Creative Commons Attribution 4.0 International License

Citation Styles

Cite this article as:
GOLDIS, A., GOLDIS, R., CORNIANU, M., BASA, N., LAZAR, D., DOBRESCU, A., LAZAR, F., Immunohistochemical Study of Cell Proliferation in Hepatocellular Carcinoma, Rev. Chim., 70(6), 2019, 2198-2203.

Vancouver
Goldis A, Goldis R, Cornianu M, Basa N, Lazar D, Dobrescu A, Lazar F. Immunohistochemical Study of Cell Proliferation in Hepatocellular Carcinoma. Rev. Chim.[internet]. 2019 Jun;70(6):2198-2203. Available from: https://doi.org/10.37358/RC.19.6.7304

APA 6th edition
Goldis, A., Goldis, R., Cornianu, M., Basa, N., Lazar, D., Dobrescu, A. & Lazar, F. (2019). Immunohistochemical Study of Cell Proliferation in Hepatocellular Carcinoma. Revista de Chimie, 70(6), 2198-2203. https://doi.org/10.37358/RC.19.6.7304

Harvard
Goldis, A., Goldis, R., Cornianu, M., Basa, N., Lazar, D., Dobrescu, A., Lazar, F. (2019). 'Immunohistochemical Study of Cell Proliferation in Hepatocellular Carcinoma', Revista de Chimie, 70(6), pp. 2198-2203. https://doi.org/10.37358/RC.19.6.7304

IEEE
A. Goldis, R. Goldis, M. Cornianu, N. Basa, D. Lazar, A. Dobrescu, F. Lazar, "Immunohistochemical Study of Cell Proliferation in Hepatocellular Carcinoma". Revista de Chimie, vol. 70, no. 6, pp. 2198-2203, 2019. [online]. https://doi.org/10.37358/RC.19.6.7304

Text
Adrian Goldis, Ramona Goldis, Marioara Cornianu, Norina Basa, Daniela Lazar, Amadeus Dobrescu, Fulger Lazar,
Immunohistochemical Study of Cell Proliferation in Hepatocellular Carcinoma,
Revista de Chimie,
Volume 70, Issue 6,
2019,
Pages 2198-2203,
ISSN 2668-8212,
https://doi.org/10.37358/RC.19.6.7304.
(https://revistadechimie.ro/Articles.asp?ID=7304)
Keywords: hepatocellular carcinoma; proliferation index of Ki-67; immunohistochemistry

RIS
TY - JOUR
T1 - Immunohistochemical Study of Cell Proliferation in Hepatocellular Carcinoma
A1 - Goldis, Adrian
A2 - Goldis, Ramona
A3 - Cornianu, Marioara
A4 - Basa, Norina
A5 - Lazar, Daniela
A6 - Dobrescu, Amadeus
A7 - Lazar, Fulger
JF - Revista de Chimie
JO - Rev. Chim.
PB - Revista de Chimie SRL
SN - 2668-8212
Y1 - 2019
VL - 70
IS - 6
SP - 2198
EP - 2203
UR - https://doi.org/10.37358/RC.19.6.7304
KW - hepatocellular carcinoma
KW - proliferation index of Ki-67
KW - immunohistochemistry
ER -

BibTex
@article{RevCh2019P2198,
author = {Goldis Adrian and Goldis Ramona and Cornianu Marioara and Basa Norina and Lazar Daniela and Dobrescu Amadeus and Lazar Fulger},
title = {Immunohistochemical Study of Cell Proliferation in Hepatocellular Carcinoma},
journal = {Revista de Chimie},
volume = {70},
number = {6},
pages = {2198-2203},
year = {2019},
issn = {2668-8212},
doi = {https://doi.org/10.37358/RC.19.6.7304},
url = {https://revistadechimie.ro/Articles.asp?ID=7304}
}

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