Evaluation of Antioxidant Capacity of Plant Extracts Using Flow Cytometry

Ionescu, Daniela; Isaia (Oarcea), Ancuta Ioana; Gafencu, Mihai; Bolintineanu, Sorin; Cata, Adina; Bratosin, Daniela; Toader, Lavinia; Ienascu, Ioana Maria Carmen

Daniela Ionescu, Ancuta Ioana Isaia (Oarcea), Mihai Gafencu, Sorin Bolintineanu, Adina Cata, Daniela Bratosin, Lavinia Toader, Ioana Maria Carmen Ienascu

Evaluation of Antioxidant Capacity of Plant Extracts Using Flow Cytometry

Abstract:

This study is aimed to evaluate the inhibitory effect of natural extracts on reactive oxygen species (ROS) in human red cells (RBCs) under oxidative stress and to demonstrate the importance of using natural extracts from plants in the fight against oxidative stress. Therefore, seven nonalcoholic extracts were tested at several dilutions, in order to prove their antioxidant activity using flow cytometry. The level of intracellular hydrogen peroxide significantly decreased when the cells were coincubated with different extracts, the values of the Mean Fluorescence Intensity (MFI) ranging from 206 to 3702, compared with 3872, the value obtained in case of H2O2-treated cells (RBCs + H2O2). All the tested extracts demonstrated a good inhibition, Melissa officinalis L. extract showed a greater percentage of hydrogen peroxide inhibition even at the last dilution (94.70% (D-a)….85.76% (D-e)). The results suggest the importance of the investigated natural extracts to combat oxidative stress.

Keywords:
plant extracts; antioxidant capacity; MFI; ROS inhibition

Issue: 2017, Volume 68, Issue 4

Pages: 652-655

Publication date: 2017/5/15

https://doi.org/10.37358/RC.17.4.5524

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This article is published under the Creative Commons Attribution 4.0 International License

Citation Styles

Cite this article as:
IONESCU, D., ISAIA (OARCEA), A.I., GAFENCU, M., BOLINTINEANU, S., CATA, A., BRATOSIN, D., TOADER, L., IENASCU, I.M.C., Evaluation of Antioxidant Capacity of Plant Extracts Using Flow Cytometry, Rev. Chim., 68(4), 2017, 652-655.

Vancouver
Ionescu D, Isaia (Oarcea) AI, Gafencu M, Bolintineanu S, Cata A, Bratosin D, Toader L, Ienascu IMC. Evaluation of Antioxidant Capacity of Plant Extracts Using Flow Cytometry. Rev. Chim.[internet]. 2017 Apr;68(4):652-655. Available from: https://doi.org/10.37358/RC.17.4.5524

APA 6th edition
Ionescu, D., Isaia (Oarcea), A.I., Gafencu, M., Bolintineanu, S., Cata, A., Bratosin, D., Toader, L. & Ienascu, I.M.C. (2017). Evaluation of Antioxidant Capacity of Plant Extracts Using Flow Cytometry. Revista de Chimie, 68(4), 652-655. https://doi.org/10.37358/RC.17.4.5524

Harvard
Ionescu, D., Isaia (Oarcea), A.I., Gafencu, M., Bolintineanu, S., Cata, A., Bratosin, D., Toader, L., Ienascu, I.M.C. (2017). 'Evaluation of Antioxidant Capacity of Plant Extracts Using Flow Cytometry', Revista de Chimie, 68(4), pp. 652-655. https://doi.org/10.37358/RC.17.4.5524

IEEE
D. Ionescu, A.I. Isaia(Oarcea), M. Gafencu, S. Bolintineanu, A. Cata, D. Bratosin, L. Toader, I.M.C. Ienascu, "Evaluation of Antioxidant Capacity of Plant Extracts Using Flow Cytometry". Revista de Chimie, vol. 68, no. 4, pp. 652-655, 2017. [online]. https://doi.org/10.37358/RC.17.4.5524

Text
Daniela Ionescu, Ancuta Ioana Isaia(Oarcea), Mihai Gafencu, Sorin Bolintineanu, Adina Cata, Daniela Bratosin, Lavinia Toader, Ioana Maria Carmen Ienascu,
Evaluation of Antioxidant Capacity of Plant Extracts Using Flow Cytometry,
Revista de Chimie,
Volume 68, Issue 4,
2017,
Pages 652-655,
ISSN 2668-8212,
https://doi.org/10.37358/RC.17.4.5524.
(https://revistadechimie.ro/Articles.asp?ID=5524)
Keywords: plant extracts; antioxidant capacity; MFI; ROS inhibition

RIS
TY - JOUR
T1 - Evaluation of Antioxidant Capacity of Plant Extracts Using Flow Cytometry
A1 - Ionescu, Daniela
A2 - Isaia (Oarcea), Ancuta Ioana
A3 - Gafencu, Mihai
A4 - Bolintineanu, Sorin
A5 - Cata, Adina
A6 - Bratosin, Daniela
A7 - Toader, Lavinia
A8 - Ienascu, Ioana Maria Carmen
JF - Revista de Chimie
JO - Rev. Chim.
PB - Revista de Chimie SRL
SN - 2668-8212
Y1 - 2017
VL - 68
IS - 4
SP - 652
EP - 655
UR - https://doi.org/10.37358/RC.17.4.5524
KW - plant extracts
KW - antioxidant capacity
KW - MFI
KW - ROS inhibition
ER -

BibTex
@article{RevCh2017P652,
author = {Ionescu Daniela and Isaia (Oarcea) Ancuta Ioana and Gafencu Mihai and Bolintineanu Sorin and Cata Adina and Bratosin Daniela and Toader Lavinia and Ienascu Ioana Maria Carmen},
title = {Evaluation of Antioxidant Capacity of Plant Extracts Using Flow Cytometry},
journal = {Revista de Chimie},
volume = {68},
number = {4},
pages = {652-655},
year = {2017},
issn = {2668-8212},
doi = {https://doi.org/10.37358/RC.17.4.5524},
url = {https://revistadechimie.ro/Articles.asp?ID=5524}
}

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