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REVISTA DE CHIMIE
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https://doi.org/10.37358/Rev.Chim.1949

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Revista de Chimie (Rev. Chim.), Year 2017, Volume 68, Issue 8, 1771-1775

https://doi.org/10.37358/RC.17.8.5762

Dumitru Petru Iga, Dumitru Popescu, Florentina Duica

The Use of Exoglycosidases for the Assay of Two New Enzymatic Substrates, b-D-xylopyranosyl-4-nitrocatechol-1-yl and a-lactosyl-4-nitrocatechol-1-yl

Abstract:

Glycosylation acceptor, 4-nitrocatechol, has been prepared via 4-nitrocatechol sulfate (2-hydroxy-5-nitrophenyl sulfate). The two carbohydrates, D-xylose and lactose, were peracetylated and then served as glycosylation donors in a modified Helferich glycosylation method, by using BF3ยท OBu2 as a promotor. The new synthesized glycosides were crystallized from ethanol and then submitted to Zémplen saponification and separated by preparative thin layer chromatography (TLC). We have isolated two xylosides. Reaction mixture of lactoside proved to be unitary, a single product could be isolated. Small portions of the synthetic glycosides were re-acetylated and their 1H and 13C NMR spectra registered. The two separated xylosides were b- and a-xylopyranoside-4-nitrocatechol-1-yl. Being submitted to the action of an enzymatic extract from digestive tract of snail (Helix pomatia) only the b-anomer was susceptible to enzymatic hydrolysis. The isolated lactoside proved to be the a-isomer. Under the action of an enzymatic extract from wheat (Triticum aestivum) germs, it was sequencially cleaved, as indicated by a kinetic TLC analysis.
Keywords:
b-D-xylopyranoside; a-D-xylopyranoside; a-lactoside; 4-nitrocatechol; b-xylosidase; b-galactosidase; a-glucosidase

Issue: 2017, Volume 68, Issue 8
Pages: 1771-1775
Publication date: 2017/9/15
https://doi.org/10.37358/RC.17.8.5762
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This article is published under the Creative Commons Attribution 4.0 International License
Citation Styles
Cite this article as:
IGA, D.P., POPESCU, D., DUICA, F., The Use of Exoglycosidases for the Assay of Two New Enzymatic Substrates, b-D-xylopyranosyl-4-nitrocatechol-1-yl and a-lactosyl-4-nitrocatechol-1-yl, Rev. Chim., 68(8), 2017, 1771-1775.

Vancouver
Iga DP, Popescu D, Duica F. The Use of Exoglycosidases for the Assay of Two New Enzymatic Substrates, b-D-xylopyranosyl-4-nitrocatechol-1-yl and a-lactosyl-4-nitrocatechol-1-yl. Rev. Chim.[internet]. 2017 Aug;68(8):1771-1775. Available from: https://doi.org/10.37358/RC.17.8.5762


APA 6th edition
Iga, D.P., Popescu, D. & Duica, F. (2017). The Use of Exoglycosidases for the Assay of Two New Enzymatic Substrates, b-D-xylopyranosyl-4-nitrocatechol-1-yl and a-lactosyl-4-nitrocatechol-1-yl. Revista de Chimie, 68(8), 1771-1775. https://doi.org/10.37358/RC.17.8.5762


Harvard
Iga, D.P., Popescu, D., Duica, F. (2017). 'The Use of Exoglycosidases for the Assay of Two New Enzymatic Substrates, b-D-xylopyranosyl-4-nitrocatechol-1-yl and a-lactosyl-4-nitrocatechol-1-yl', Revista de Chimie, 68(8), pp. 1771-1775. https://doi.org/10.37358/RC.17.8.5762


IEEE
D.P. Iga, D. Popescu, F. Duica, "The Use of Exoglycosidases for the Assay of Two New Enzymatic Substrates, b-D-xylopyranosyl-4-nitrocatechol-1-yl and a-lactosyl-4-nitrocatechol-1-yl". Revista de Chimie, vol. 68, no. 8, pp. 1771-1775, 2017. [online]. https://doi.org/10.37358/RC.17.8.5762


Text
Dumitru Petru Iga, Dumitru Popescu, Florentina Duica,
The Use of Exoglycosidases for the Assay of Two New Enzymatic Substrates, b-D-xylopyranosyl-4-nitrocatechol-1-yl and a-lactosyl-4-nitrocatechol-1-yl,
Revista de Chimie,
Volume 68, Issue 8,
2017,
Pages 1771-1775,
ISSN 2668-8212,
https://doi.org/10.37358/RC.17.8.5762.
(https://revistadechimie.ro/Articles.asp?ID=5762)
Keywords: b-D-xylopyranoside; a-D-xylopyranoside; a-lactoside; 4-nitrocatechol; b-xylosidase; b-galactosidase; a-glucosidase


RIS
TY - JOUR
T1 - The Use of Exoglycosidases for the Assay of Two New Enzymatic Substrates, b-D-xylopyranosyl-4-nitrocatechol-1-yl and a-lactosyl-4-nitrocatechol-1-yl
A1 - Iga, Dumitru Petru
A2 - Popescu, Dumitru
A3 - Duica, Florentina
JF - Revista de Chimie
JO - Rev. Chim.
PB - Revista de Chimie SRL
SN - 2668-8212
Y1 - 2017
VL - 68
IS - 8
SP - 1771
EP - 1775
UR - https://doi.org/10.37358/RC.17.8.5762
KW - b-D-xylopyranoside
KW - a-D-xylopyranoside
KW - a-lactoside
KW - 4-nitrocatechol
KW - b-xylosidase
KW - b-galactosidase
KW - a-glucosidase
ER -


BibTex
@article{RevCh2017P1771,
author = {Iga Dumitru Petru and Popescu Dumitru and Duica Florentina},
title = {The Use of Exoglycosidases for the Assay of Two New Enzymatic Substrates, b-D-xylopyranosyl-4-nitrocatechol-1-yl and a-lactosyl-4-nitrocatechol-1-yl},
journal = {Revista de Chimie},
volume = {68},
number = {8},
pages = {1771-1775},
year = {2017},
issn = {2668-8212},
doi = {https://doi.org/10.37358/RC.17.8.5762},
url = {https://revistadechimie.ro/Articles.asp?ID=5762}
}
 
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