Invertase Production by Fungi, Characterization of Enzyme Activity and Kinetic Parameters

Matei, Gabi Mirela; Matei, Sorin; Pele, Maria; Dumitrescu, Flavia; Matei, Adrian

Gabi Mirela Matei, Sorin Matei, Maria Pele, Flavia Dumitrescu, Adrian Matei

Invertase Production by Fungi, Characterization of Enzyme Activity and Kinetic Parameters

Abstract:

The present paper presents the results of research carried out on 14 fungal isolates of various origins aiming to select new efficient sources for invertase production for further biotechnological application. Aspergillus flavus presented the highest protein content and Aspergillus niger, the most intense invertase activity. The relationship between enzyme concentration and enzymatic activity at 0.25 mM mL-1sucrose as substrate assayed for successive decimal dilutions of Aspergillus niger enzyme ranging from 0.1 to 1mlLrevealed a linear correspondence between 0.1 and 0.5mL. The kinetic parameters Michaelis-Menten constant (Km) and maximal velocity (Vmax) for invertase activity of Aspergillus niger, Penicillium aurantiogriseum, Aspergillus wentii and Rhizopus stolonifer were calculated. The determination coefficients R2 calculated from Lineweaver-Burk plots presented values very close or equal to 1.

Keywords:
invertase; filamentous fungi; sucrose; kinetic parameters; proteins

Issue: 2017, Volume 68, Issue 10

Pages: 2205-2208

Publication date: 2017/11/15

https://doi.org/10.37358/RC.17.10.5856

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This article is published under the Creative Commons Attribution 4.0 International License

Citation Styles

Cite this article as:
MATEI, G.M., MATEI, S., PELE, M., DUMITRESCU, F., MATEI, A., Invertase Production by Fungi, Characterization of Enzyme Activity and Kinetic Parameters, Rev. Chim., 68(10), 2017, 2205-2208.

Vancouver
Matei GM, Matei S, Pele M, Dumitrescu F, Matei A. Invertase Production by Fungi, Characterization of Enzyme Activity and Kinetic Parameters. Rev. Chim.[internet]. 2017 Oct;68(10):2205-2208. Available from: https://doi.org/10.37358/RC.17.10.5856

APA 6th edition
Matei, G.M., Matei, S., Pele, M., Dumitrescu, F. & Matei, A. (2017). Invertase Production by Fungi, Characterization of Enzyme Activity and Kinetic Parameters. Revista de Chimie, 68(10), 2205-2208. https://doi.org/10.37358/RC.17.10.5856

Harvard
Matei, G.M., Matei, S., Pele, M., Dumitrescu, F., Matei, A. (2017). 'Invertase Production by Fungi, Characterization of Enzyme Activity and Kinetic Parameters', Revista de Chimie, 68(10), pp. 2205-2208. https://doi.org/10.37358/RC.17.10.5856

IEEE
G.M. Matei, S. Matei, M. Pele, F. Dumitrescu, A. Matei, "Invertase Production by Fungi, Characterization of Enzyme Activity and Kinetic Parameters". Revista de Chimie, vol. 68, no. 10, pp. 2205-2208, 2017. [online]. https://doi.org/10.37358/RC.17.10.5856

Text
Gabi Mirela Matei, Sorin Matei, Maria Pele, Flavia Dumitrescu, Adrian Matei,
Invertase Production by Fungi, Characterization of Enzyme Activity and Kinetic Parameters,
Revista de Chimie,
Volume 68, Issue 10,
2017,
Pages 2205-2208,
ISSN 2668-8212,
https://doi.org/10.37358/RC.17.10.5856.
(https://revistadechimie.ro/Articles.asp?ID=5856)
Keywords: invertase; filamentous fungi; sucrose; kinetic parameters; proteins

RIS
TY - JOUR
T1 - Invertase Production by Fungi, Characterization of Enzyme Activity and Kinetic Parameters
A1 - Matei, Gabi Mirela
A2 - Matei, Sorin
A3 - Pele, Maria
A4 - Dumitrescu, Flavia
A5 - Matei, Adrian
JF - Revista de Chimie
JO - Rev. Chim.
PB - Revista de Chimie SRL
SN - 2668-8212
Y1 - 2017
VL - 68
IS - 10
SP - 2205
EP - 2208
UR - https://doi.org/10.37358/RC.17.10.5856
KW - invertase
KW - filamentous fungi
KW - sucrose
KW - kinetic parameters
KW - proteins
ER -

BibTex
@article{RevCh2017P2205,
author = {Matei Gabi Mirela and Matei Sorin and Pele Maria and Dumitrescu Flavia and Matei Adrian},
title = {Invertase Production by Fungi, Characterization of Enzyme Activity and Kinetic Parameters},
journal = {Revista de Chimie},
volume = {68},
number = {10},
pages = {2205-2208},
year = {2017},
issn = {2668-8212},
doi = {https://doi.org/10.37358/RC.17.10.5856},
url = {https://revistadechimie.ro/Articles.asp?ID=5856}
}

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