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REVISTA DE CHIMIE
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https://doi.org/10.37358/Rev.Chim.1949

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Revista de Chimie (Rev. Chim.), Year 2017, Volume 68, Issue 10, 2389-2396

https://doi.org/10.37358/RC.17.10.5892

Khoukhi Faiza, Salima Kebouche, Nacer Eddine Djelali, Mohamed Lamine Gana

The Application of Enzymological Techniques for Determination of the Inhibitor Activity of Bio Enzym Extracted from Seawater Bacteria Pseudomonas aerogenosa to Fight Biocorrosion of Carbon Steel

Abstract:

The paper presents a studies for the development of new corrosion inhibitors by biotechnological way applicable to many areas of petroleum industry including, characterization of protective films on carbon steel and biocorrosion inhibition. Our research is oriented towards the isolation of new strains of bacteria from the sea waters of the Mediterranean region that have power (inhibitory and/or bactericidal that blocks the growth of sulfate-reducing bacteria responsible for microbiologically influenced by production of Pyocyanin (1 hydroxyphenazin) or 5-methylphenazin-1-one. Our first objective was to isolate the population of Pseudomonas Aerugenosa on a specific medium, characterization gram, then fermentation in a nutrient broth. The second objective of this study is evaluation of corrosion rate by weight loss method with injecting different dose of the crude enzyme extract CEE containing the methylphenazine (Pyocyanin). An efficacy test was performed on test kit vials containing the specific culture medium with the SRB contaminated with industrial injection water from SONAHESS region. The total disappearance of the SRB is 40% of CEE by blocking their metabolism in the growth phase confirms the efficiency of treatment.
Keywords:
Biotechnology; Pseudomonas aeruginosa; fermentation; enzymology; bactericide; biocorrosion; nutrient broth; inhibition; sulfate-reducing bacteria

Issue: 2017, Volume 68, Issue 10
Pages: 2389-2396
Publication date: 2017/11/15
https://doi.org/10.37358/RC.17.10.5892
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This article is published under the Creative Commons Attribution 4.0 International License
Citation Styles
Cite this article as:
FAIZA, K., KEBOUCHE, S., DJELALI, N.E., GANA, M.L., The Application of Enzymological Techniques for Determination of the Inhibitor Activity of Bio Enzym Extracted from Seawater Bacteria Pseudomonas aerogenosa to Fight Biocorrosion of Carbon Steel, Rev. Chim., 68(10), 2017, 2389-2396.

Vancouver
Faiza K, Kebouche S, Djelali NE, Gana ML. The Application of Enzymological Techniques for Determination of the Inhibitor Activity of Bio Enzym Extracted from Seawater Bacteria Pseudomonas aerogenosa to Fight Biocorrosion of Carbon Steel. Rev. Chim.[internet]. 2017 Oct;68(10):2389-2396. Available from: https://doi.org/10.37358/RC.17.10.5892


APA 6th edition
Faiza, K., Kebouche, S., Djelali, N.E. & Gana, M.L. (2017). The Application of Enzymological Techniques for Determination of the Inhibitor Activity of Bio Enzym Extracted from Seawater Bacteria Pseudomonas aerogenosa to Fight Biocorrosion of Carbon Steel. Revista de Chimie, 68(10), 2389-2396. https://doi.org/10.37358/RC.17.10.5892


Harvard
Faiza, K., Kebouche, S., Djelali, N.E., Gana, M.L. (2017). 'The Application of Enzymological Techniques for Determination of the Inhibitor Activity of Bio Enzym Extracted from Seawater Bacteria Pseudomonas aerogenosa to Fight Biocorrosion of Carbon Steel', Revista de Chimie, 68(10), pp. 2389-2396. https://doi.org/10.37358/RC.17.10.5892


IEEE
K. Faiza, S. Kebouche, N.E. Djelali, M.L. Gana, "The Application of Enzymological Techniques for Determination of the Inhibitor Activity of Bio Enzym Extracted from Seawater Bacteria Pseudomonas aerogenosa to Fight Biocorrosion of Carbon Steel". Revista de Chimie, vol. 68, no. 10, pp. 2389-2396, 2017. [online]. https://doi.org/10.37358/RC.17.10.5892


Text
Khoukhi Faiza, Salima Kebouche, Nacer Eddine Djelali, Mohamed Lamine Gana,
The Application of Enzymological Techniques for Determination of the Inhibitor Activity of Bio Enzym Extracted from Seawater Bacteria Pseudomonas aerogenosa to Fight Biocorrosion of Carbon Steel,
Revista de Chimie,
Volume 68, Issue 10,
2017,
Pages 2389-2396,
ISSN 2668-8212,
https://doi.org/10.37358/RC.17.10.5892.
(https://revistadechimie.ro/Articles.asp?ID=5892)
Keywords: Biotechnology; Pseudomonas aeruginosa; fermentation; enzymology; bactericide; biocorrosion; nutrient broth; inhibition; sulfate-reducing bacteria


RIS
TY - JOUR
T1 - The Application of Enzymological Techniques for Determination of the Inhibitor Activity of Bio Enzym Extracted from Seawater Bacteria Pseudomonas aerogenosa to Fight Biocorrosion of Carbon Steel
A1 - Faiza, Khoukhi
A2 - Kebouche, Salima
A3 - Djelali, Nacer Eddine
A4 - Gana, Mohamed Lamine
JF - Revista de Chimie
JO - Rev. Chim.
PB - Revista de Chimie SRL
SN - 2668-8212
Y1 - 2017
VL - 68
IS - 10
SP - 2389
EP - 2396
UR - https://doi.org/10.37358/RC.17.10.5892
KW - Biotechnology
KW - Pseudomonas aeruginosa
KW - fermentation
KW - enzymology
KW - bactericide
KW - biocorrosion
KW - nutrient broth
KW - inhibition
KW - sulfate-reducing bacteria
ER -


BibTex
@article{RevCh2017P2389,
author = {Faiza Khoukhi and Kebouche Salima and Djelali Nacer Eddine and Gana Mohamed Lamine},
title = {The Application of Enzymological Techniques for Determination of the Inhibitor Activity of Bio Enzym Extracted from Seawater Bacteria Pseudomonas aerogenosa to Fight Biocorrosion of Carbon Steel},
journal = {Revista de Chimie},
volume = {68},
number = {10},
pages = {2389-2396},
year = {2017},
issn = {2668-8212},
doi = {https://doi.org/10.37358/RC.17.10.5892},
url = {https://revistadechimie.ro/Articles.asp?ID=5892}
}
 
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