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REVISTA DE CHIMIE
Cite as: Rev. Chim.
https://doi.org/10.37358/Rev.Chim.1949

OSIM Nr. R102355
ISSN Online 2668-8212
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Revista de Chimie (Rev. Chim.), Year 2017, Volume 68, Issue 12, 2853-2856

https://doi.org/10.37358/RC.17.12.5993

Igor Jelihovschi, Cristian Drochioi, Aida Corina Badescu, Raoul Vasile Lupusoru, Alexandra Elena Munteanu, Nicolae Baranov, Daniela Manuc, Raluca Ioana Serban, Roxana Gabriela Cobzaru, Carmen Valerica Ripa, Luminita Smaranda Iancu

Comparison of Sampling Techniques For qPCR Quantification of Periodontal Pathogens

Abstract:

The diagnosis of periodontal disease is mainly based on use of clinical and radiographic evidence. In this study we employed a quantitative PCR analysis of Aggregatibacter actinomycetemcomitans and Treponema denticola as species strongly involved in periodontal diseases, burden in periodontal pockets to detect the main sampling factors that interfere with qPCR results. From 22 patients with advanced periodontal disease, subgingival plaque was comparatively collected by paper points and periodontal Gracey curettes. Samples were collected from the same situs in presence of gingival bleeding and absence of bleeding. The concordance and agreement of results between samples were assessed. The present study demonstrates that subgingival plaque sampling with sterile absorbable paper points is often accompanied by gingival bleeding resulting in quantification biases of periodontal pathogens.
Keywords:
periodontitis; qPCR; diagnosis; sampling

Issue: 2017, Volume 68, Issue 12
Pages: 2853-2856
Publication date: 2018/1/15
https://doi.org/10.37358/RC.17.12.5993
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Creative Commons License
This article is published under the Creative Commons Attribution 4.0 International License
Citation Styles
Cite this article as:
JELIHOVSCHI, I., DROCHIOI, C., BADESCU, A.C., LUPUSORU, R.V., MUNTEANU, A.E., BARANOV, N., MANUC, D., SERBAN, R.I., COBZARU, R.G., RIPA, C.V., IANCU, L.S., Comparison of Sampling Techniques For qPCR Quantification of Periodontal Pathogens, Rev. Chim., 68(12), 2017, 2853-2856.

Vancouver
Jelihovschi I, Drochioi C, Badescu AC, Lupusoru RV, Munteanu AE, Baranov N, Manuc D, Serban RI, Cobzaru RG, Ripa CV, Iancu LS. Comparison of Sampling Techniques For qPCR Quantification of Periodontal Pathogens. Rev. Chim.[internet]. 2017 Dec;68(12):2853-2856. Available from: https://doi.org/10.37358/RC.17.12.5993


APA 6th edition
Jelihovschi, I., Drochioi, C., Badescu, A.C., Lupusoru, R.V., Munteanu, A.E., Baranov, N.,... Iancu, L.S. (2017). Comparison of Sampling Techniques For qPCR Quantification of Periodontal Pathogens. Revista de Chimie, 68(12), 2853-2856. https://doi.org/10.37358/RC.17.12.5993


Harvard
Jelihovschi, I., Drochioi, C., Badescu, A.C., Lupusoru, R.V., Munteanu, A.E., Baranov, N., Manuc, D., Serban, R.I., Cobzaru, R.G., Ripa, C.V., Iancu, L.S. (2017). 'Comparison of Sampling Techniques For qPCR Quantification of Periodontal Pathogens', Revista de Chimie, 68(12), pp. 2853-2856. https://doi.org/10.37358/RC.17.12.5993


IEEE
I. Jelihovschi, C. Drochioi, A.C. Badescu, R.V. Lupusoru, A.E. Munteanu, N. Baranov, D. Manuc, R.I. Serban, R.G. Cobzaru, C.V. Ripa, L.S. Iancu, "Comparison of Sampling Techniques For qPCR Quantification of Periodontal Pathogens". Revista de Chimie, vol. 68, no. 12, pp. 2853-2856, 2017. [online]. https://doi.org/10.37358/RC.17.12.5993


Text
Igor Jelihovschi, Cristian Drochioi, Aida Corina Badescu, Raoul Vasile Lupusoru, Alexandra Elena Munteanu, Nicolae Baranov, Daniela Manuc, Raluca Ioana Serban, Roxana Gabriela Cobzaru, Carmen Valerica Ripa, Luminita Smaranda Iancu,
Comparison of Sampling Techniques For qPCR Quantification of Periodontal Pathogens,
Revista de Chimie,
Volume 68, Issue 12,
2017,
Pages 2853-2856,
ISSN 2668-8212,
https://doi.org/10.37358/RC.17.12.5993.
(https://revistadechimie.ro/Articles.asp?ID=5993)
Keywords: periodontitis; qPCR; diagnosis; sampling


RIS
TY - JOUR
T1 - Comparison of Sampling Techniques For qPCR Quantification of Periodontal Pathogens
A1 - Jelihovschi, Igor
A2 - Drochioi, Cristian
A3 - Badescu, Aida Corina
A4 - Lupusoru, Raoul Vasile
A5 - Munteanu, Alexandra Elena
A6 - Baranov, Nicolae
A7 - Manuc, Daniela
A8 - Serban, Raluca Ioana
A9 - Cobzaru, Roxana Gabriela
A10 - Ripa, Carmen Valerica
A11 - Iancu, Luminita Smaranda
JF - Revista de Chimie
JO - Rev. Chim.
PB - Revista de Chimie SRL
SN - 2668-8212
Y1 - 2017
VL - 68
IS - 12
SP - 2853
EP - 2856
UR - https://doi.org/10.37358/RC.17.12.5993
KW - periodontitis
KW - qPCR
KW - diagnosis
KW - sampling
ER -


BibTex
@article{RevCh2017P2853,
author = {Jelihovschi Igor and Drochioi Cristian and Badescu Aida Corina and Lupusoru Raoul Vasile and Munteanu Alexandra Elena and Baranov Nicolae and Manuc Daniela and Serban Raluca Ioana and Cobzaru Roxana Gabriela and Ripa Carmen Valerica and Iancu Luminita Smaranda},
title = {Comparison of Sampling Techniques For qPCR Quantification of Periodontal Pathogens},
journal = {Revista de Chimie},
volume = {68},
number = {12},
pages = {2853-2856},
year = {2017},
issn = {2668-8212},
doi = {https://doi.org/10.37358/RC.17.12.5993},
url = {https://revistadechimie.ro/Articles.asp?ID=5993}
}
 
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