Dimethyl-sulfoxide is a Suitable Solvent for Fluorescent Microscopy Detection of Medium and Strong Heat Shock Inductors Using Transgenic Zebrafish

Preluca, Vlad; Serb, Bogdan Horatiu; Marchian, Sanda; Atasie, Diter; Mitariu, Mihaela Cernusca; Sabau, Alexandru; Tantar, Cristian; Sabau, Dan

Vlad Preluca, Bogdan Horatiu Serb, Sanda Marchian, Diter Atasie, Mihaela Cernusca Mitariu, Alexandru Sabau, Cristian Tantar, Dan Sabau

Dimethyl-sulfoxide is a Suitable Solvent for Fluorescent Microscopy Detection of Medium and Strong Heat Shock Inductors Using Transgenic Zebrafish

Abstract:

Heat shock inductors have potential as treatment for degenerative and protein misfolding diseases. Dimethyl-sulfoxide is widely used as a solvent in pharmacological screening tests and has been shown to have heat shock induction effects. Transgenic Tg (hsp70l:EGFP-HRAS_G12V)io3(AB) zebrafish larvae were exposed for 24 hours to dimethyl-sulfoxide in concentratios of 0.1-2%, and to moderate heat shock inductors pentoxifylline and tacrolimus. Positive controls were exposed to 35, 38 and 40°C for 20 min, and incubated for 24 h at 28°C. Heat shock response was measured by fluorescence microscopy and signal intensity quantification in FIJI. Dimethyl-sulfoxide caused a dose-dependant increase in fluorescent intensity, but significantly lower compared with exposure to 38 and 40°C. Pentoxifylline and tacrolimus induced a significantly higher increase in fluorescence compared with 0.5% dimethyl-sulfoxide. Thus, although dimethyl-sulfoxide has independent heat shock induction effects, concentrations of up to 0.5% are suitable for heat shock response screening tests.

Keywords:
dimethyl-sulfoxide; heat shock response; zebrafish

Issue: 2018, Volume 69, Issue 2

Pages: 337-340

Publication date: 2018/3/15

https://doi.org/10.37358/RC.18.2.6102

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This article is published under the Creative Commons Attribution 4.0 International License

Citation Styles

Cite this article as:
PRELUCA, V., SERB, B.H., MARCHIAN, S., ATASIE, D., MITARIU, M.C., SABAU, A., TANTAR, C., SABAU, D., Dimethyl-sulfoxide is a Suitable Solvent for Fluorescent Microscopy Detection of Medium and Strong Heat Shock Inductors Using Transgenic Zebrafish, Rev. Chim., 69(2), 2018, 337-340.

Vancouver
Preluca V, Serb BH, Marchian S, Atasie D, Mitariu MC, Sabau A, Tantar C, Sabau D. Dimethyl-sulfoxide is a Suitable Solvent for Fluorescent Microscopy Detection of Medium and Strong Heat Shock Inductors Using Transgenic Zebrafish. Rev. Chim.[internet]. 2018 Feb;69(2):337-340. Available from: https://doi.org/10.37358/RC.18.2.6102

APA 6th edition
Preluca, V., Serb, B.H., Marchian, S., Atasie, D., Mitariu, M.C., Sabau, A., Tantar, C. & Sabau, D. (2018). Dimethyl-sulfoxide is a Suitable Solvent for Fluorescent Microscopy Detection of Medium and Strong Heat Shock Inductors Using Transgenic Zebrafish. Revista de Chimie, 69(2), 337-340. https://doi.org/10.37358/RC.18.2.6102

Harvard
Preluca, V., Serb, B.H., Marchian, S., Atasie, D., Mitariu, M.C., Sabau, A., Tantar, C., Sabau, D. (2018). 'Dimethyl-sulfoxide is a Suitable Solvent for Fluorescent Microscopy Detection of Medium and Strong Heat Shock Inductors Using Transgenic Zebrafish', Revista de Chimie, 69(2), pp. 337-340. https://doi.org/10.37358/RC.18.2.6102

IEEE
V. Preluca, B.H. Serb, S. Marchian, D. Atasie, M.C. Mitariu, A. Sabau, C. Tantar, D. Sabau, "Dimethyl-sulfoxide is a Suitable Solvent for Fluorescent Microscopy Detection of Medium and Strong Heat Shock Inductors Using Transgenic Zebrafish". Revista de Chimie, vol. 69, no. 2, pp. 337-340, 2018. [online]. https://doi.org/10.37358/RC.18.2.6102

Text
Vlad Preluca, Bogdan Horatiu Serb, Sanda Marchian, Diter Atasie, Mihaela Cernusca Mitariu, Alexandru Sabau, Cristian Tantar, Dan Sabau,
Dimethyl-sulfoxide is a Suitable Solvent for Fluorescent Microscopy Detection of Medium and Strong Heat Shock Inductors Using Transgenic Zebrafish,
Revista de Chimie,
Volume 69, Issue 2,
2018,
Pages 337-340,
ISSN 2668-8212,
https://doi.org/10.37358/RC.18.2.6102.
(https://revistadechimie.ro/Articles.asp?ID=6102)
Keywords: dimethyl-sulfoxide; heat shock response; zebrafish

RIS
TY - JOUR
T1 - Dimethyl-sulfoxide is a Suitable Solvent for Fluorescent Microscopy Detection of Medium and Strong Heat Shock Inductors Using Transgenic Zebrafish
A1 - Preluca, Vlad
A2 - Serb, Bogdan Horatiu
A3 - Marchian, Sanda
A4 - Atasie, Diter
A5 - Mitariu, Mihaela Cernusca
A6 - Sabau, Alexandru
A7 - Tantar, Cristian
A8 - Sabau, Dan
JF - Revista de Chimie
JO - Rev. Chim.
PB - Revista de Chimie SRL
SN - 2668-8212
Y1 - 2018
VL - 69
IS - 2
SP - 337
EP - 340
UR - https://doi.org/10.37358/RC.18.2.6102
KW - dimethyl-sulfoxide
KW - heat shock response
KW - zebrafish
ER -

BibTex
@article{RevCh2018P337,
author = {Preluca Vlad and Serb Bogdan Horatiu and Marchian Sanda and Atasie Diter and Mitariu Mihaela Cernusca and Sabau Alexandru and Tantar Cristian and Sabau Dan},
title = {Dimethyl-sulfoxide is a Suitable Solvent for Fluorescent Microscopy Detection of Medium and Strong Heat Shock Inductors Using Transgenic Zebrafish},
journal = {Revista de Chimie},
volume = {69},
number = {2},
pages = {337-340},
year = {2018},
issn = {2668-8212},
doi = {https://doi.org/10.37358/RC.18.2.6102},
url = {https://revistadechimie.ro/Articles.asp?ID=6102}
}

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