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REVISTA DE CHIMIE
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https://doi.org/10.37358/Rev.Chim.1949

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Revista de Chimie (Rev. Chim.), Year 2018, Volume 69, Issue 3, 645-649

https://doi.org/10.37358/RC.18.3.6167

Dan Spinu, Ovidiu Bratu, Dragos Marcu, Dan Mischianu, Radu Huica, Mihaela Surcel, Adriana Munteanu, Bogdan Socea, Oana Bodean, Cornel Ursaciuc

The Use of ELISA and PCR in Identifying Correlations between Viral Infection and Benign Prostatic Hypertrophy

Abstract:

Benign prostatic hyperplasia (BPH) is induced by a persistent local inflammatory process that leads to cell proliferation. Viral infections associated with immune deficiencies can trigger the chronic inflammation of the prostate. Therefore, we have investigated several viral expressions in BPH patients and tried to establish a link with the diagnosed hyperplasia. 50 patients with BPH without urinary tract infection were tested for the presence of the following viruses: human papilloma virus (HPV), cytomegalovirus (CMV) and Epstein-Barr virus (EBV). These viruses are the most common cause of asymptomatic viral infections. HPV-specific DNA detection by polymerase chain reaction (PCR) was used for freshly surgical removed tissue sample. Both anti-CMV (IgG, IgM) and anti-EBV (IgG, IgM) antibodies were detected in the patients’ serum with standard enzyme-Linked Immunosorbent Assay technique (ELISA). Specific HPV-DNA in prostate tissue was found only in 4% of patients, while 98% and 100% patients were positive for serum anti-CMV IgG or anti-EBV, proving intense earlier contact with the virus. IgM anti-CMV evaluation was found in around 10% of the cases which were also negative for EBV, sustaining that this was a non-acute infection. The findings showed that BPH may be associated with a chronic inflammation due to the post-viral infection with CMV or EBV, or secondary to the presence of these viruses in the prostate, while the involvement of HPV infection in BPH development is comparably lower. Our data suggests that viral investigation in BPH should be considered in the screening protocol of BPH as an indicator of possible inflammatory-mediated tumorigenesis of urinary tract.
Keywords:
benign prostatic hypertrophy; human papilloma virus; cytomegalovirus; Epstein-Barr virus

Issue: 2018, Volume 69, Issue 3
Pages: 645-649
Publication date: 2018/4/15
https://doi.org/10.37358/RC.18.3.6167
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Creative Commons License
This article is published under the Creative Commons Attribution 4.0 International License
Citation Styles
Cite this article as:
SPINU, D., BRATU, O., MARCU, D., MISCHIANU, D., HUICA, R., SURCEL, M., MUNTEANU, A., SOCEA, B., BODEAN, O., URSACIUC, C., The Use of ELISA and PCR in Identifying Correlations between Viral Infection and Benign Prostatic Hypertrophy, Rev. Chim., 69(3), 2018, 645-649.

Vancouver
Spinu D, Bratu O, Marcu D, Mischianu D, Huica R, Surcel M, Munteanu A, Socea B, Bodean O, Ursaciuc C. The Use of ELISA and PCR in Identifying Correlations between Viral Infection and Benign Prostatic Hypertrophy. Rev. Chim.[internet]. 2018 Mar;69(3):645-649. Available from: https://doi.org/10.37358/RC.18.3.6167


APA 6th edition
Spinu, D., Bratu, O., Marcu, D., Mischianu, D., Huica, R., Surcel, M.,... Ursaciuc, C. (2018). The Use of ELISA and PCR in Identifying Correlations between Viral Infection and Benign Prostatic Hypertrophy. Revista de Chimie, 69(3), 645-649. https://doi.org/10.37358/RC.18.3.6167


Harvard
Spinu, D., Bratu, O., Marcu, D., Mischianu, D., Huica, R., Surcel, M., Munteanu, A., Socea, B., Bodean, O., Ursaciuc, C. (2018). 'The Use of ELISA and PCR in Identifying Correlations between Viral Infection and Benign Prostatic Hypertrophy', Revista de Chimie, 69(3), pp. 645-649. https://doi.org/10.37358/RC.18.3.6167


IEEE
D. Spinu, O. Bratu, D. Marcu, D. Mischianu, R. Huica, M. Surcel, A. Munteanu, B. Socea, O. Bodean, C. Ursaciuc, "The Use of ELISA and PCR in Identifying Correlations between Viral Infection and Benign Prostatic Hypertrophy". Revista de Chimie, vol. 69, no. 3, pp. 645-649, 2018. [online]. https://doi.org/10.37358/RC.18.3.6167


Text
Dan Spinu, Ovidiu Bratu, Dragos Marcu, Dan Mischianu, Radu Huica, Mihaela Surcel, Adriana Munteanu, Bogdan Socea, Oana Bodean, Cornel Ursaciuc,
The Use of ELISA and PCR in Identifying Correlations between Viral Infection and Benign Prostatic Hypertrophy,
Revista de Chimie,
Volume 69, Issue 3,
2018,
Pages 645-649,
ISSN 2668-8212,
https://doi.org/10.37358/RC.18.3.6167.
(https://revistadechimie.ro/Articles.asp?ID=6167)
Keywords: benign prostatic hypertrophy; human papilloma virus; cytomegalovirus; Epstein-Barr virus


RIS
TY - JOUR
T1 - The Use of ELISA and PCR in Identifying Correlations between Viral Infection and Benign Prostatic Hypertrophy
A1 - Spinu, Dan
A2 - Bratu, Ovidiu
A3 - Marcu, Dragos
A4 - Mischianu, Dan
A5 - Huica, Radu
A6 - Surcel, Mihaela
A7 - Munteanu, Adriana
A8 - Socea, Bogdan
A9 - Bodean, Oana
A10 - Ursaciuc, Cornel
JF - Revista de Chimie
JO - Rev. Chim.
PB - Revista de Chimie SRL
SN - 2668-8212
Y1 - 2018
VL - 69
IS - 3
SP - 645
EP - 649
UR - https://doi.org/10.37358/RC.18.3.6167
KW - benign prostatic hypertrophy
KW - human papilloma virus
KW - cytomegalovirus
KW - Epstein-Barr virus
ER -


BibTex
@article{RevCh2018P645,
author = {Spinu Dan and Bratu Ovidiu and Marcu Dragos and Mischianu Dan and Huica Radu and Surcel Mihaela and Munteanu Adriana and Socea Bogdan and Bodean Oana and Ursaciuc Cornel},
title = {The Use of ELISA and PCR in Identifying Correlations between Viral Infection and Benign Prostatic Hypertrophy},
journal = {Revista de Chimie},
volume = {69},
number = {3},
pages = {645-649},
year = {2018},
issn = {2668-8212},
doi = {https://doi.org/10.37358/RC.18.3.6167},
url = {https://revistadechimie.ro/Articles.asp?ID=6167}
}
 
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