Assessment of Giardia lamblia Infection Prevalence Using Lugol’s Iodine Staining Technique

Olariu, Tudor Rares; Mazilu, Octavian; Mihaescu, Rodica; Ududec, Titus; Timar, Romulus; Bolintineanu, Sorin; Petrescu, Cristina; Gurban, Camelia Vidita

Tudor Rares Olariu, Octavian Mazilu, Rodica Mihaescu, Titus Ududec, Romulus Timar, Sorin Bolintineanu, Cristina Petrescu, Camelia Vidita Gurban

Assessment of Giardia lamblia Infection Prevalence Using Lugol’s Iodine Staining Technique

Abstract:

Parasitic diseases have a worldwide distribution and represent an important public health problem. The aim of the present study was to evaluate the prevalence of intestinal parasitic infections in a Child Day Care Center from Western Romania using direct wet mount and concentration procedures of stool samples. One hundred sixty-four children aged 2-8 years were investigated. Stool examinations were performed using the iodine staining for the identification of protozoan cysts and the Willis-Hung concentration method for the identification of helminth eggs. Routine laboratory investigations were also conducted to evaluate the eosinophil count in the infected children. Intestinal parasitic infections were diagnosed in 47 cases (28.6%). Giardia lamblia (25%), Entamoeba coli (5.5%), Blastocystis hominis (1.2%), Ascaris lumbricoides (0.6%) and Trichuris trichiura (0.6%) were the only parasites identified. Among the children with parasitic infections we have determined association of two parasites in 7 (14.9%). We have found that in children with giardiasis the eosinophil values were increased (5.54±4.21%) compared to controls (3.69±1.48%) (p[0.01). Our findings suggest that giardiasis may be significantly prevalent in children attending day care centers in Western Romania. Efficient educational programs should be timely implemented in child care centers with the aim of providing appropriate infection control measures.

Keywords:
iodine staining; concentration methods; giardiasis; protozoan; parasite

Issue: 2018, Volume 69, Issue 3

Pages: 665-667

Publication date: 2018/4/15

https://doi.org/10.37358/RC.18.3.6171

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This article is published under the Creative Commons Attribution 4.0 International License

Citation Styles

Cite this article as:
OLARIU, T.R., MAZILU, O., MIHAESCU, R., UDUDEC, T., TIMAR, R., BOLINTINEANU, S., PETRESCU, C., GURBAN, C.V., Assessment of Giardia lamblia Infection Prevalence Using Lugol’s Iodine Staining Technique, Rev. Chim., 69(3), 2018, 665-667.

Vancouver
Olariu TR, Mazilu O, Mihaescu R, Ududec T, Timar R, Bolintineanu S, Petrescu C, Gurban CV. Assessment of Giardia lamblia Infection Prevalence Using Lugol’s Iodine Staining Technique. Rev. Chim.[internet]. 2018 Mar;69(3):665-667. Available from: https://doi.org/10.37358/RC.18.3.6171

APA 6th edition
Olariu, T.R., Mazilu, O., Mihaescu, R., Ududec, T., Timar, R., Bolintineanu, S., Petrescu, C. & Gurban, C.V. (2018). Assessment of Giardia lamblia Infection Prevalence Using Lugol’s Iodine Staining Technique. Revista de Chimie, 69(3), 665-667. https://doi.org/10.37358/RC.18.3.6171

Harvard
Olariu, T.R., Mazilu, O., Mihaescu, R., Ududec, T., Timar, R., Bolintineanu, S., Petrescu, C., Gurban, C.V. (2018). 'Assessment of Giardia lamblia Infection Prevalence Using Lugol’s Iodine Staining Technique', Revista de Chimie, 69(3), pp. 665-667. https://doi.org/10.37358/RC.18.3.6171

IEEE
T.R. Olariu, O. Mazilu, R. Mihaescu, T. Ududec, R. Timar, S. Bolintineanu, C. Petrescu, C.V. Gurban, "Assessment of Giardia lamblia Infection Prevalence Using Lugol’s Iodine Staining Technique". Revista de Chimie, vol. 69, no. 3, pp. 665-667, 2018. [online]. https://doi.org/10.37358/RC.18.3.6171

Text
Tudor Rares Olariu, Octavian Mazilu, Rodica Mihaescu, Titus Ududec, Romulus Timar, Sorin Bolintineanu, Cristina Petrescu, Camelia Vidita Gurban,
Assessment of Giardia lamblia Infection Prevalence Using Lugol’s Iodine Staining Technique,
Revista de Chimie,
Volume 69, Issue 3,
2018,
Pages 665-667,
ISSN 2668-8212,
https://doi.org/10.37358/RC.18.3.6171.
(https://revistadechimie.ro/Articles.asp?ID=6171)
Keywords: iodine staining; concentration methods; giardiasis; protozoan; parasite

RIS
TY - JOUR
T1 - Assessment of Giardia lamblia Infection Prevalence Using Lugol’s Iodine Staining Technique
A1 - Olariu, Tudor Rares
A2 - Mazilu, Octavian
A3 - Mihaescu, Rodica
A4 - Ududec, Titus
A5 - Timar, Romulus
A6 - Bolintineanu, Sorin
A7 - Petrescu, Cristina
A8 - Gurban, Camelia Vidita
JF - Revista de Chimie
JO - Rev. Chim.
PB - Revista de Chimie SRL
SN - 2668-8212
Y1 - 2018
VL - 69
IS - 3
SP - 665
EP - 667
UR - https://doi.org/10.37358/RC.18.3.6171
KW - iodine staining
KW - concentration methods
KW - giardiasis
KW - protozoan
KW - parasite
ER -

BibTex
@article{RevCh2018P665,
author = {Olariu Tudor Rares and Mazilu Octavian and Mihaescu Rodica and Ududec Titus and Timar Romulus and Bolintineanu Sorin and Petrescu Cristina and Gurban Camelia Vidita},
title = {Assessment of Giardia lamblia Infection Prevalence Using Lugol’s Iodine Staining Technique},
journal = {Revista de Chimie},
volume = {69},
number = {3},
pages = {665-667},
year = {2018},
issn = {2668-8212},
doi = {https://doi.org/10.37358/RC.18.3.6171},
url = {https://revistadechimie.ro/Articles.asp?ID=6171}
}

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