Characterization of the Eugenol Effects on the Bioenergetic Profile of SCC-4 Human Squamous Cell Carcinoma Cell Line

Duicu, Oana M.; Pavel, Ioana Z.; Borcan, Florin; Muntean, Danina M.; Cheveresan, Adelina; Bratu, Emanuel A.; Rusu, Laura Cristina; Karancsi, Olimpiu Ladislau

Oana M. Duicu, Ioana Z. Pavel, Florin Borcan, Danina M. Muntean, Adelina Cheveresan, Emanuel A. Bratu, Laura Cristina Rusu, Olimpiu Ladislau Karancsi

Characterization of the Eugenol Effects on the Bioenergetic Profile of SCC-4 Human Squamous Cell Carcinoma Cell Line

Abstract:

Eugenol (EU), the active ingredient in clove oil, is commonly used as successful therapeutic compound in dentistry due to its antiseptic and anti-inflammatory effects. Recent research studies suggest that eugenol has also a potential anti-cancer effect. This study was thereby purported to assess the effects of EU on the bioenergetic profile of the SCC-4 human squamous cell carcinoma cell line. To this aim, SCC-4 cells were treated for 24 hours with free EU and EU incorporated in polyurethane structures (50 µM each). Oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) were measured using the Seahorse XF-24e extracellular flux analyzer (Agilent Technologies Inc.). Analysis of the SCC-4 bioenergetic profile was performed in the presence of the classic modulators of the electron transport chain: oligomycin, FCCP, and antimycin A+rotenone. Our data showed that cells stimulated with free EU induced a decrease of OCR linked parameters and an increase of ECAR, effects that were abolished by the incorporation of EU in polyurethane structures. In conclusion, free eugenol elicits inhibitory effects on mitochondrial respiration in the SCC-4 cell line, a result that might be suggestive for its anti-tumoral effects.

Keywords:
eugenol; SCC-4 human squamous cell carcinoma; mitochondrial function; cellular bioenergetics; glycolysis

Issue: 2018, Volume 69, Issue 9

Pages: 2567-2570

Publication date: 2018/10/15

https://doi.org/10.37358/RC.18.9.6577

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This article is published under the Creative Commons Attribution 4.0 International License

Citation Styles

Cite this article as:
DUICU, O.M., PAVEL, I.Z., BORCAN, F., MUNTEAN, D.M., CHEVERESAN, A., BRATU, E.A., RUSU, L.C., KARANCSI, O.L., Characterization of the Eugenol Effects on the Bioenergetic Profile of SCC-4 Human Squamous Cell Carcinoma Cell Line, Rev. Chim., 69(9), 2018, 2567-2570.

Vancouver
Duicu OM, Pavel IZ, Borcan F, Muntean DM, Cheveresan A, Bratu EA, Rusu LC, Karancsi OL. Characterization of the Eugenol Effects on the Bioenergetic Profile of SCC-4 Human Squamous Cell Carcinoma Cell Line. Rev. Chim.[internet]. 2018 Sep;69(9):2567-2570. Available from: https://doi.org/10.37358/RC.18.9.6577

APA 6th edition
Duicu, O.M., Pavel, I.Z., Borcan, F., Muntean, D.M., Cheveresan, A., Bratu, E.A., Rusu, L.C. & Karancsi, O.L. (2018). Characterization of the Eugenol Effects on the Bioenergetic Profile of SCC-4 Human Squamous Cell Carcinoma Cell Line. Revista de Chimie, 69(9), 2567-2570. https://doi.org/10.37358/RC.18.9.6577

Harvard
Duicu, O.M., Pavel, I.Z., Borcan, F., Muntean, D.M., Cheveresan, A., Bratu, E.A., Rusu, L.C., Karancsi, O.L. (2018). 'Characterization of the Eugenol Effects on the Bioenergetic Profile of SCC-4 Human Squamous Cell Carcinoma Cell Line', Revista de Chimie, 69(9), pp. 2567-2570. https://doi.org/10.37358/RC.18.9.6577

IEEE
O.M. Duicu, I.Z. Pavel, F. Borcan, D.M. Muntean, A. Cheveresan, E.A. Bratu, L.C. Rusu, O.L. Karancsi, "Characterization of the Eugenol Effects on the Bioenergetic Profile of SCC-4 Human Squamous Cell Carcinoma Cell Line". Revista de Chimie, vol. 69, no. 9, pp. 2567-2570, 2018. [online]. https://doi.org/10.37358/RC.18.9.6577

Text
Oana M. Duicu, Ioana Z. Pavel, Florin Borcan, Danina M. Muntean, Adelina Cheveresan, Emanuel A. Bratu, Laura Cristina Rusu, Olimpiu Ladislau Karancsi,
Characterization of the Eugenol Effects on the Bioenergetic Profile of SCC-4 Human Squamous Cell Carcinoma Cell Line,
Revista de Chimie,
Volume 69, Issue 9,
2018,
Pages 2567-2570,
ISSN 2668-8212,
https://doi.org/10.37358/RC.18.9.6577.
(https://revistadechimie.ro/Articles.asp?ID=6577)
Keywords: eugenol; SCC-4 human squamous cell carcinoma; mitochondrial function; cellular bioenergetics; glycolysis

RIS
TY - JOUR
T1 - Characterization of the Eugenol Effects on the Bioenergetic Profile of SCC-4 Human Squamous Cell Carcinoma Cell Line
A1 - Duicu, Oana M.
A2 - Pavel, Ioana Z.
A3 - Borcan, Florin
A4 - Muntean, Danina M.
A5 - Cheveresan, Adelina
A6 - Bratu, Emanuel A.
A7 - Rusu, Laura Cristina
A8 - Karancsi, Olimpiu Ladislau
JF - Revista de Chimie
JO - Rev. Chim.
PB - Revista de Chimie SRL
SN - 2668-8212
Y1 - 2018
VL - 69
IS - 9
SP - 2567
EP - 2570
UR - https://doi.org/10.37358/RC.18.9.6577
KW - eugenol
KW - SCC-4 human squamous cell carcinoma
KW - mitochondrial function
KW - cellular bioenergetics
KW - glycolysis
ER -

BibTex
@article{RevCh2018P2567,
author = {Duicu Oana M. and Pavel Ioana Z. and Borcan Florin and Muntean Danina M. and Cheveresan Adelina and Bratu Emanuel A. and Rusu Laura Cristina and Karancsi Olimpiu Ladislau},
title = {Characterization of the Eugenol Effects on the Bioenergetic Profile of SCC-4 Human Squamous Cell Carcinoma Cell Line},
journal = {Revista de Chimie},
volume = {69},
number = {9},
pages = {2567-2570},
year = {2018},
issn = {2668-8212},
doi = {https://doi.org/10.37358/RC.18.9.6577},
url = {https://revistadechimie.ro/Articles.asp?ID=6577}
}

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