Studies on Chromatographic Fractioning by Cations Exchangers of a Bovine Hemoglobin Hydrolysate

Panainte, Alina Diana; Morariu, Ionela Daniela; Bibire, Nela; Vieriu, Madalina; Tantaru, Gladiola; Luca, Alina Costina; Apostu, Mihai

Alina Diana Panainte, Ionela Daniela Morariu, Nela Bibire, Madalina Vieriu, Gladiola Tantaru, Alina Costina Luca, Mihai Apostu

Studies on Chromatographic Fractioning by Cations Exchangers of a Bovine Hemoglobin Hydrolysate

Abstract:

A peptidic hydrolysate has been obtained through hydrolysis of bovine hemoglobin using pepsin. The fractioning of the hydrolysate was performed on a column packed with CM-Sepharose Fast Flow. The hydrolysate and each fraction was filtered and then injected into a HPLC system equipped with a Vydak C4 reverse phase column (0.46 x 25 cm), suitable for the chromatographic separation of large peptides with 20 to 30 amino acids. The detection was done using mass spectrometry, and the retention time, size and distribution of the peptides were determined.

Keywords:
hemoglobin; cation exchangers; RP-HPLC

Issue: 2018, Volume 69, Issue 10

Pages: 2794-2798

Publication date: 2018/11/15

https://doi.org/10.37358/RC.18.10.6626

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This article is published under the Creative Commons Attribution 4.0 International License

Citation Styles

Cite this article as:
PANAINTE, A.D., MORARIU, I.D., BIBIRE, N., VIERIU, M., TANTARU, G., LUCA, A.C., APOSTU, M., Studies on Chromatographic Fractioning by Cations Exchangers of a Bovine Hemoglobin Hydrolysate, Rev. Chim., 69(10), 2018, 2794-2798.

Vancouver
Panainte AD, Morariu ID, Bibire N, Vieriu M, Tantaru G, Luca AC, Apostu M. Studies on Chromatographic Fractioning by Cations Exchangers of a Bovine Hemoglobin Hydrolysate. Rev. Chim.[internet]. 2018 Oct;69(10):2794-2798. Available from: https://doi.org/10.37358/RC.18.10.6626

APA 6th edition
Panainte, A.D., Morariu, I.D., Bibire, N., Vieriu, M., Tantaru, G., Luca, A.C. & Apostu, M. (2018). Studies on Chromatographic Fractioning by Cations Exchangers of a Bovine Hemoglobin Hydrolysate. Revista de Chimie, 69(10), 2794-2798. https://doi.org/10.37358/RC.18.10.6626

Harvard
Panainte, A.D., Morariu, I.D., Bibire, N., Vieriu, M., Tantaru, G., Luca, A.C., Apostu, M. (2018). 'Studies on Chromatographic Fractioning by Cations Exchangers of a Bovine Hemoglobin Hydrolysate', Revista de Chimie, 69(10), pp. 2794-2798. https://doi.org/10.37358/RC.18.10.6626

IEEE
A.D. Panainte, I.D. Morariu, N. Bibire, M. Vieriu, G. Tantaru, A.C. Luca, M. Apostu, "Studies on Chromatographic Fractioning by Cations Exchangers of a Bovine Hemoglobin Hydrolysate". Revista de Chimie, vol. 69, no. 10, pp. 2794-2798, 2018. [online]. https://doi.org/10.37358/RC.18.10.6626

Text
Alina Diana Panainte, Ionela Daniela Morariu, Nela Bibire, Madalina Vieriu, Gladiola Tantaru, Alina Costina Luca, Mihai Apostu,
Studies on Chromatographic Fractioning by Cations Exchangers of a Bovine Hemoglobin Hydrolysate,
Revista de Chimie,
Volume 69, Issue 10,
2018,
Pages 2794-2798,
ISSN 2668-8212,
https://doi.org/10.37358/RC.18.10.6626.
(https://revistadechimie.ro/Articles.asp?ID=6626)
Keywords: hemoglobin; cation exchangers; RP-HPLC

RIS
TY - JOUR
T1 - Studies on Chromatographic Fractioning by Cations Exchangers of a Bovine Hemoglobin Hydrolysate
A1 - Panainte, Alina Diana
A2 - Morariu, Ionela Daniela
A3 - Bibire, Nela
A4 - Vieriu, Madalina
A5 - Tantaru, Gladiola
A6 - Luca, Alina Costina
A7 - Apostu, Mihai
JF - Revista de Chimie
JO - Rev. Chim.
PB - Revista de Chimie SRL
SN - 2668-8212
Y1 - 2018
VL - 69
IS - 10
SP - 2794
EP - 2798
UR - https://doi.org/10.37358/RC.18.10.6626
KW - hemoglobin
KW - cation exchangers
KW - RP-HPLC
ER -

BibTex
@article{RevCh2018P2794,
author = {Panainte Alina Diana and Morariu Ionela Daniela and Bibire Nela and Vieriu Madalina and Tantaru Gladiola and Luca Alina Costina and Apostu Mihai},
title = {Studies on Chromatographic Fractioning by Cations Exchangers of a Bovine Hemoglobin Hydrolysate},
journal = {Revista de Chimie},
volume = {69},
number = {10},
pages = {2794-2798},
year = {2018},
issn = {2668-8212},
doi = {https://doi.org/10.37358/RC.18.10.6626},
url = {https://revistadechimie.ro/Articles.asp?ID=6626}
}

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