Reversed-Phase High Performance Liquid Chromatographic Analysis of Three First Line Anti-Tuberculosis Drugs

Bibire, Nela; Olariu, Romeo Iulian; Agoroaei, Luminita; Vieriu, Madalina; Panainte, Alina Diana; Cernat, Roberta Ionela; Arsene, Cecilia

Nela Bibire, Romeo Iulian Olariu, Luminita Agoroaei, Madalina Vieriu, Alina Diana Panainte, Roberta Ionela Cernat, Cecilia Arsene

Reversed-Phase High Performance Liquid Chromatographic Analysis of Three First Line Anti-Tuberculosis Drugs

Abstract:

Active pharmaceutical ingredients such as isoniazid, pyrazinamide and rifampicin are among the most important first-line anti-tuberculosis drugs. A simple, rapid and sensitive reversed phase-high performance liquid chromatographic assay method for the simultaneous determination of isoniazid, pyrazinamide and rifampicin has been developed. Separation of the interest compounds was achieved in a 10 min chromatographic run in gradient elution mode on a Zorbax SB-C18 stainless steel column (150 × 4 mm, 5 mm) using a guard column containing the same stationary phase. The gradient elution was carried out with a mobile phase of 10% CH3CN aqueous solution for channel A and 50% CH3CN in pH = 6.8 phosphate buffer (20 mM), to which 1.5 mL triethylamine were added for channel B. Quantification of the analyzed substances was carried out spectrophotometrically at 269 nm. Detection limits of 0.48 mg/L for isoniazid, 0.52 mg/L for pyrazinamide and 0.48 mg/L for rifampicin were established for the developed assay method. The present work showed that the proposed analysis method was advantageous for simple and rapid analysis of the active pharmaceutical ingredients in pharmaceuticals and biological fluids.

Keywords:
isoniazid; pyrazinamide; rifampicin; first-line anti-tuberculosis drugs; HPLC

Issue: 2018, Volume 69, Issue 12

Pages: 3590-3592

Publication date: 2019/1/15

https://doi.org/10.37358/RC.18.12.6799

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This article is published under the Creative Commons Attribution 4.0 International License

Citation Styles

Cite this article as:
BIBIRE, N., OLARIU, R.I., AGOROAEI, L., VIERIU, M., PANAINTE, A.D., CERNAT, R.I., ARSENE, C., Reversed-Phase High Performance Liquid Chromatographic Analysis of Three First Line Anti-Tuberculosis Drugs, Rev. Chim., 69(12), 2018, 3590-3592.

Vancouver
Bibire N, Olariu RI, Agoroaei L, Vieriu M, Panainte AD, Cernat RI, Arsene C. Reversed-Phase High Performance Liquid Chromatographic Analysis of Three First Line Anti-Tuberculosis Drugs. Rev. Chim.[internet]. 2018 Dec;69(12):3590-3592. Available from: https://doi.org/10.37358/RC.18.12.6799

APA 6th edition
Bibire, N., Olariu, R.I., Agoroaei, L., Vieriu, M., Panainte, A.D., Cernat, R.I. & Arsene, C. (2018). Reversed-Phase High Performance Liquid Chromatographic Analysis of Three First Line Anti-Tuberculosis Drugs. Revista de Chimie, 69(12), 3590-3592. https://doi.org/10.37358/RC.18.12.6799

Harvard
Bibire, N., Olariu, R.I., Agoroaei, L., Vieriu, M., Panainte, A.D., Cernat, R.I., Arsene, C. (2018). 'Reversed-Phase High Performance Liquid Chromatographic Analysis of Three First Line Anti-Tuberculosis Drugs', Revista de Chimie, 69(12), pp. 3590-3592. https://doi.org/10.37358/RC.18.12.6799

IEEE
N. Bibire, R.I. Olariu, L. Agoroaei, M. Vieriu, A.D. Panainte, R.I. Cernat, C. Arsene, "Reversed-Phase High Performance Liquid Chromatographic Analysis of Three First Line Anti-Tuberculosis Drugs". Revista de Chimie, vol. 69, no. 12, pp. 3590-3592, 2018. [online]. https://doi.org/10.37358/RC.18.12.6799

Text
Nela Bibire, Romeo Iulian Olariu, Luminita Agoroaei, Madalina Vieriu, Alina Diana Panainte, Roberta Ionela Cernat, Cecilia Arsene,
Reversed-Phase High Performance Liquid Chromatographic Analysis of Three First Line Anti-Tuberculosis Drugs,
Revista de Chimie,
Volume 69, Issue 12,
2018,
Pages 3590-3592,
ISSN 2668-8212,
https://doi.org/10.37358/RC.18.12.6799.
(https://revistadechimie.ro/Articles.asp?ID=6799)
Keywords: isoniazid; pyrazinamide; rifampicin; first-line anti-tuberculosis drugs; HPLC

RIS
TY - JOUR
T1 - Reversed-Phase High Performance Liquid Chromatographic Analysis of Three First Line Anti-Tuberculosis Drugs
A1 - Bibire, Nela
A2 - Olariu, Romeo Iulian
A3 - Agoroaei, Luminita
A4 - Vieriu, Madalina
A5 - Panainte, Alina Diana
A6 - Cernat, Roberta Ionela
A7 - Arsene, Cecilia
JF - Revista de Chimie
JO - Rev. Chim.
PB - Revista de Chimie SRL
SN - 2668-8212
Y1 - 2018
VL - 69
IS - 12
SP - 3590
EP - 3592
UR - https://doi.org/10.37358/RC.18.12.6799
KW - isoniazid
KW - pyrazinamide
KW - rifampicin
KW - first-line anti-tuberculosis drugs
KW - HPLC
ER -

BibTex
@article{RevCh2018P3590,
author = {Bibire Nela and Olariu Romeo Iulian and Agoroaei Luminita and Vieriu Madalina and Panainte Alina Diana and Cernat Roberta Ionela and Arsene Cecilia},
title = {Reversed-Phase High Performance Liquid Chromatographic Analysis of Three First Line Anti-Tuberculosis Drugs},
journal = {Revista de Chimie},
volume = {69},
number = {12},
pages = {3590-3592},
year = {2018},
issn = {2668-8212},
doi = {https://doi.org/10.37358/RC.18.12.6799},
url = {https://revistadechimie.ro/Articles.asp?ID=6799}
}

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