Microscopic and Molecular Detection of Nosema ceranae in Honeybee Apis mellifera L. from Romania Status on pathogen worldwide distribution

Mederle, Narcisa; Lobo, Maria Luisa; Morariu, Sorin; Morariu, Florica; Darabus, Gheorghe; Mederle, Ovidiu; Matos, Olga

Narcisa Mederle, Maria Luisa Lobo, Sorin Morariu, Florica Morariu, Gheorghe Darabus, Ovidiu Mederle, Olga Matos

Microscopic and Molecular Detection of Nosema ceranae in Honeybee Apis mellifera L. from Romania Status on pathogen worldwide distribution

Abstract:

Until now, in Romania, a country in southeastern Europe located on the Balkans Peninsula, information regarding the status on A. mellifera honey bee infection by Nosema spp. has not been published. The aims of the present study were to survey the occurrence and identification of Nosema spp. in A. mellifera honeybee colonies from 37 apiaries located in Arad, Caras-Severin and Timis Counties, Romania. Also, based on published literature an update on the distribution of N. ceranae infection among A. mellifera colonies worldwide was performed. Overall, a frequency of 55.1% (389/690) of Nosema infection was observed in the analyzed regions (ranging from 52% to 66%), by light microscopy. By PCR and DNA sequencing, N. ceranae was the only microsporidia identified. Nosema ceranae is clearly a novel, emergent pathogen of A.mellifera with potentially very serious effects on the individual and honeybee’s colonies in Romania. Data obtained provide new and important information on N. ceranae geographic prevalence and distribution, and on its impact at colony level and/or its role in colony losses. The present study intends to contribute to highlight the importance of implementing prevention, treatment and control measures of honeybee nosemosis, in Romania.

Keywords:
Apis mellifera honeybee; Microsporidia; Nosemosis; Nosema ceranae; Molecular characterization

Issue: 2018, Volume 69, Issue 12

Pages: 3761-3772

Publication date: 2019/1/15

https://doi.org/10.37358/RC.18.12.6837

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This article is published under the Creative Commons Attribution 4.0 International License

Citation Styles

Cite this article as:
MEDERLE, N., LOBO, M.L., MORARIU, S., MORARIU, F., DARABUS, G., MEDERLE, O., MATOS, O., Microscopic and Molecular Detection of Nosema ceranae in Honeybee Apis mellifera L. from Romania Status on pathogen worldwide distribution, Rev. Chim., 69(12), 2018, 3761-3772.

Vancouver
Mederle N, Lobo ML, Morariu S, Morariu F, Darabus G, Mederle O, Matos O. Microscopic and Molecular Detection of Nosema ceranae in Honeybee Apis mellifera L. from Romania Status on pathogen worldwide distribution. Rev. Chim.[internet]. 2018 Dec;69(12):3761-3772. Available from: https://doi.org/10.37358/RC.18.12.6837

APA 6th edition
Mederle, N., Lobo, M.L., Morariu, S., Morariu, F., Darabus, G., Mederle, O. & Matos, O. (2018). Microscopic and Molecular Detection of Nosema ceranae in Honeybee Apis mellifera L. from Romania Status on pathogen worldwide distribution. Revista de Chimie, 69(12), 3761-3772. https://doi.org/10.37358/RC.18.12.6837

Harvard
Mederle, N., Lobo, M.L., Morariu, S., Morariu, F., Darabus, G., Mederle, O., Matos, O. (2018). 'Microscopic and Molecular Detection of Nosema ceranae in Honeybee Apis mellifera L. from Romania Status on pathogen worldwide distribution', Revista de Chimie, 69(12), pp. 3761-3772. https://doi.org/10.37358/RC.18.12.6837

IEEE
N. Mederle, M.L. Lobo, S. Morariu, F. Morariu, G. Darabus, O. Mederle, O. Matos, "Microscopic and Molecular Detection of Nosema ceranae in Honeybee Apis mellifera L. from Romania Status on pathogen worldwide distribution". Revista de Chimie, vol. 69, no. 12, pp. 3761-3772, 2018. [online]. https://doi.org/10.37358/RC.18.12.6837

Text
Narcisa Mederle, Maria Luisa Lobo, Sorin Morariu, Florica Morariu, Gheorghe Darabus, Ovidiu Mederle, Olga Matos,
Microscopic and Molecular Detection of Nosema ceranae in Honeybee Apis mellifera L. from Romania Status on pathogen worldwide distribution,
Revista de Chimie,
Volume 69, Issue 12,
2018,
Pages 3761-3772,
ISSN 2668-8212,
https://doi.org/10.37358/RC.18.12.6837.
(https://revistadechimie.ro/Articles.asp?ID=6837)
Keywords: Apis mellifera honeybee; Microsporidia; Nosemosis; Nosema ceranae; Molecular characterization

RIS
TY - JOUR
T1 - Microscopic and Molecular Detection of Nosema ceranae in Honeybee Apis mellifera L. from Romania Status on pathogen worldwide distribution
A1 - Mederle, Narcisa
A2 - Lobo, Maria Luisa
A3 - Morariu, Sorin
A4 - Morariu, Florica
A5 - Darabus, Gheorghe
A6 - Mederle, Ovidiu
A7 - Matos, Olga
JF - Revista de Chimie
JO - Rev. Chim.
PB - Revista de Chimie SRL
SN - 2668-8212
Y1 - 2018
VL - 69
IS - 12
SP - 3761
EP - 3772
UR - https://doi.org/10.37358/RC.18.12.6837
KW - Apis mellifera honeybee; Microsporidia; Nosemosis; Nosema ceranae; Molecular characterization
ER -

BibTex
@article{RevCh2018P3761,
author = {Mederle Narcisa and Lobo Maria Luisa and Morariu Sorin and Morariu Florica and Darabus Gheorghe and Mederle Ovidiu and Matos Olga},
title = {Microscopic and Molecular Detection of Nosema ceranae in Honeybee Apis mellifera L. from Romania Status on pathogen worldwide distribution},
journal = {Revista de Chimie},
volume = {69},
number = {12},
pages = {3761-3772},
year = {2018},
issn = {2668-8212},
doi = {https://doi.org/10.37358/RC.18.12.6837},
url = {https://revistadechimie.ro/Articles.asp?ID=6837}
}

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