The Mutation Profiles of KRAS and BRAF Genes in a Romanian Colorectal Cancer Cohort

Brinzan, Costel; Aschie, Mariana; Grasa, Catalin Nicolae; Mitroi, Anca Florentina; Matei, Elena; Cozaru, Georgeta Camelia

Costel Brinzan, Mariana Aschie, Catalin Nicolae Grasa, Anca Florentina Mitroi, Elena Matei, Georgeta Camelia Cozaru

The Mutation Profiles of KRAS and BRAF Genes in a Romanian Colorectal Cancer Cohort

Abstract:

Colorectal cancer (CRC) is one of most commonly diagnosed malignancies and management of CRC differs in according with patient’s characteristics, tumor type, differentiation, metastatic extension and KRAS/BRAF mutations. Based on this knowledge, we examined the relationship between KRAS/BRAF mutations in paraffin-embedded tumor specimens and some clinicopathological features at CRC in order to provide reliable results to the oncologists and so to contribute to the best care provided to the patients. A 56 of colorectal cancer samples were analyzed for the KRAS and BRAF mutational status using StripAssay method from ViennaLab, Austria. Assays for identification of KRAS/BRAF mutations were based on polymerase chain reaction (PCR) and reverse-hybridization. KRAS mutations were present in 50% (28 patients) of all analyzed CRC and were located in codons 12, 13 and 61. The most frequent types of mutations were substitution of glycine to valine in codon 12 (c.35G]T; 9/28), followed by glycine to aspartate on codon 13 (c.38G]A; 5/28). BRAF mutations were detected at 9 patients (16%) and in all cases Val600Glu mutation has been observed. In one case we reported a concomitant KRAS/BRAF mutation. According with current data, KRAS and BRAF mutations are associated with a poor patient prognosis in CRC, but KRAS mutation in codon 13 and BRAF appear to have a higher oncogenic potential.

Keywords:
colorectal cancer; KRAS; BRAF; mutation; genotype

Issue: 2019, Volume 70, Issue 4

Pages: 1346-1350

Publication date: 2019/5/15

https://doi.org/10.37358/RC.19.4.7124

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This article is published under the Creative Commons Attribution 4.0 International License

Citation Styles

Cite this article as:
BRINZAN, C., ASCHIE, M., GRASA, C.N., MITROI, A.F., MATEI, E., COZARU, G.C., The Mutation Profiles of KRAS and BRAF Genes in a Romanian Colorectal Cancer Cohort, Rev. Chim., 70(4), 2019, 1346-1350.

Vancouver
Brinzan C, Aschie M, Grasa CN, Mitroi AF, Matei E, Cozaru GC. The Mutation Profiles of KRAS and BRAF Genes in a Romanian Colorectal Cancer Cohort. Rev. Chim.[internet]. 2019 Apr;70(4):1346-1350. Available from: https://doi.org/10.37358/RC.19.4.7124

APA 6th edition
Brinzan, C., Aschie, M., Grasa, C.N., Mitroi, A.F., Matei, E. & Cozaru, G.C. (2019). The Mutation Profiles of KRAS and BRAF Genes in a Romanian Colorectal Cancer Cohort. Revista de Chimie, 70(4), 1346-1350. https://doi.org/10.37358/RC.19.4.7124

Harvard
Brinzan, C., Aschie, M., Grasa, C.N., Mitroi, A.F., Matei, E., Cozaru, G.C. (2019). 'The Mutation Profiles of KRAS and BRAF Genes in a Romanian Colorectal Cancer Cohort', Revista de Chimie, 70(4), pp. 1346-1350. https://doi.org/10.37358/RC.19.4.7124

IEEE
C. Brinzan, M. Aschie, C.N. Grasa, A.F. Mitroi, E. Matei, G.C. Cozaru, "The Mutation Profiles of KRAS and BRAF Genes in a Romanian Colorectal Cancer Cohort". Revista de Chimie, vol. 70, no. 4, pp. 1346-1350, 2019. [online]. https://doi.org/10.37358/RC.19.4.7124

Text
Costel Brinzan, Mariana Aschie, Catalin Nicolae Grasa, Anca Florentina Mitroi, Elena Matei, Georgeta Camelia Cozaru,
The Mutation Profiles of KRAS and BRAF Genes in a Romanian Colorectal Cancer Cohort,
Revista de Chimie,
Volume 70, Issue 4,
2019,
Pages 1346-1350,
ISSN 2668-8212,
https://doi.org/10.37358/RC.19.4.7124.
(https://revistadechimie.ro/Articles.asp?ID=7124)
Keywords: colorectal cancer; KRAS; BRAF; mutation; genotype

RIS
TY - JOUR
T1 - The Mutation Profiles of KRAS and BRAF Genes in a Romanian Colorectal Cancer Cohort
A1 - Brinzan, Costel
A2 - Aschie, Mariana
A3 - Grasa, Catalin Nicolae
A4 - Mitroi, Anca Florentina
A5 - Matei, Elena
A6 - Cozaru, Georgeta Camelia
JF - Revista de Chimie
JO - Rev. Chim.
PB - Revista de Chimie SRL
SN - 2668-8212
Y1 - 2019
VL - 70
IS - 4
SP - 1346
EP - 1350
UR - https://doi.org/10.37358/RC.19.4.7124
KW - colorectal cancer
KW - KRAS
KW - BRAF
KW - mutation
KW - genotype
ER -

BibTex
@article{RevCh2019P1346,
author = {Brinzan Costel and Aschie Mariana and Grasa Catalin Nicolae and Mitroi Anca Florentina and Matei Elena and Cozaru Georgeta Camelia},
title = {The Mutation Profiles of KRAS and BRAF Genes in a Romanian Colorectal Cancer Cohort},
journal = {Revista de Chimie},
volume = {70},
number = {4},
pages = {1346-1350},
year = {2019},
issn = {2668-8212},
doi = {https://doi.org/10.37358/RC.19.4.7124},
url = {https://revistadechimie.ro/Articles.asp?ID=7124}
}

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