CEZAR COMANESCU, DENISA FICAI, IOANA LACATUSU, CORNELIA GURAN NEW ORGANIC – INORGANIC HYBRID MATERIALS FOR ENZYMES IMMOBILIZATION
The purpose of this study is the preparation and characterization of organic-inorganic hybrid materials used for enzyme immobilization on silica-based support by cross-linking method. The cross-linking agent used was glutaraldehyde and the enzyme used for immobilization was lipase. Organic-inorganic hybrid materials were obtained by the sol-gel process in the presence of different surfactant systems. A nanosized silica-based support was synthesized and characterized by dynamic light scattering and scanning microscopy analysis. The modifications in the chemical composition of the organic-inorganic hybrid materials before and after lipase immobilization were analyzed by infrared spectroscopy. The amount of immobilized lipase (Lowry method) and the enzymatic activity were determined.