VALERIA RADULESCU, CRINA SAVIUC, CARMEN CHIFIRIUC, ELIZA OPREA, DIANA CAROLINA ILIES, LUMINITA MARUTESCU, VERONICA LAZAR CHEMICAL COMPOSITION AND ANTIMICROBIAL ACTIVITY OF ESSENTIAL OIL FROM SHOOTS SPRUCE (PICEA ABIES L)
The aim of this paper was to evaluate the chemical composition, antimicrobial properties of volatile oil isolated from sprouts of Picea abies growing wild in Romania. The essential oil from young sprouts of Picea abies L. obtained by hidrodistillation was analysed by gas chromatography-mass spectrometry (GC-MS). Fifty four compounds accounting for 96.30 – 98.42% of the oil were identified. The main compounds found belong to monoterpenic hydrocarbons (a-pinene, camphene, limonene, myrcene), oxigenated monoterpenes (bornyl acetate), sesquiterpene hydrocarbons (d-cadinene, muurolene), sesquiterpenic alcohols (cadinol, muurolol) and diterpenic alcohol (manool). The antimicrobial activity of the volatile oil was qualitatively tested against Gram-positive and Gram-negative bacteria and fungi. The susceptible bacterial strains were assessed by microdilution technique for minimal inhibitory concentration (MIC) values. The essential oil extracted from Picea abies possess antimicrobial activity of different intensity depending on the tested strains and in some cases on the solvent used for diluting the oil. The most evident inhibitory effect was noticed against the Gram-positive and fungi strains.