MARIANA FERDES, CAMELIA UNGUREANU STUDY OF RELATIONSHIP BETWEEN ENZYMES PRODUCTION, GROWTH RATE AND PIGMENTOGENESIS FOR FIVE MUTANT STRAINS OF MONASCUS RUBER
Five mutant strains of Monascus ruber were obtained by gamma and e-beam processing and were characterized in terms of enzyme production, growth rate and pigmentogenesis. These strains were cultivated onto glucose-yeast extract agar slants for maintenance and on rice medium for pigments biosynthesis. It was demonstrated that the parental strain does not produce any amylolytic, lypolytic, proteolytic or cellulolytic enzymes, while mutant strains have enzymatic activity. The biosynthesis of natural pigments was achieved in solid state fermentation on sterilized rice. The biopigments were extracted in ethanol and hexane and the absorption spectra were recorded for the five mutant strains. M5 and M2 mutants produced the highest amount of red and yellow pigments.
Keywords: Monascus ruber, gamma and e-beam irrradiation, enzymes