ALEXANDRU OGODESCU, ATTILA ALEXANDRU MORVAY, EMILIA OGODESCU, LAURA CRISTINA RUSU, CLAUDIA SALA, IRINA ZETU, LAVINIA ARDELEAN, CRISTINA BRATU VISUALIZATION OF RESIN PENETRATION INTO ENAMEL CARIES LESIONS OF TEMPORARY TEETH – A CONFOCAL MICROSCOPIC STUDY
Carious enamel infiltration with a low viscous light cured resin (Infiltrant) is an innovative and promising approach to arrest these lesions. The confocal laser scanning microscopy (CLSM) can be used to analyze the penetration of the Infiltrant into the enamel. Most studies were performed on permanent teeth but given the structural differences to temporary teeth several aspects regarding their enamel infiltration must be elucidated. In additional it should be borne in mind that children have limited patience, so reducing the treatment time is desirable. Purpose of this study was to visualize the penetration of a low viscosity resin (Icon Infiltrant, DMG, Hamburg, Germany) into natural caries lesions of temporary teeth after 2 min of application time. Exfoliated human deciduous teeth with proximal non-cavitated enamel caries lesions were infiltrated for 2 min and then light-cured, respecting the classic therapeutic procedures proposed by the German company. The visualization of the resin infiltration was accomplished using the indirect staining protocol described by Sebastian Paris et al in 2009. Lesion depths (LD) and penetration depths (PD) were assessed using dual fluorescence confocal microscopy. This technique appears to be feasible in the case of deciduous teeth, can be used in further studies and an application time of 2 minutes was sufficient in most cases.
Key-Words: temporary teeth, infiltration of carious enamel, infiltrant, CLSM, white spot lesions