IOAN CALINESCU, PETRE CHIPURICI, ADRIAN TRIFAN, ELVIRA ALEXANDRESCU, ROMULUS DIMA, CORINA TEIU SACCHAROMYCES CEREVISIAE IMMOBILIZATION IN POLYACRYLAMIDE CRYOGEL – ACTIVATION AND TESTING IN CONTINUOUS REACTOR
Immobilized yeast on polyacrylamide cryogel was evaluated for the continuously production of ethanol using molasses as carbon source in filled bed reactor. This paper will focus on the polyacrylamide gel supports obtained at low temperature, a condition that allows obtaining a structure with large pores and specific surface. The activation of the supported yeast before use is of great importance. A polyacrylamide support containing 2% dry yeast was tested to investigate the growth and proliferation of immobilized yeast cells in the time of activation. For this study gravimetric and scanning electron microscopy was used. The activity of the immobilized yeast was tested in a continuous fermentation reactor to underline the influence of the molasses concentration, the molasses solution flow, the quantity of support filled into the reactor and the fermentation temperature over the ethanol concentration, productivity and yield process.