GEORGETA PAVALACHE, VASILE DORNEANU, ANTOANELA POPESCU A NEW HPLC METHOD FOR THE SEPARATION AND QUANTITATIVE DETERMINATION OF LORATADINE
The development of a HPLC method for determination of an antihistaminic H1 drug, last generation, loratadine, in different samples (pharmaceuticals, biological environments etc.). The method involved liquid – phase extraction of loratadine using methanol as solvent. In isocratic conditions as follows: type-C8 chromatography column (Zorbax Eclipse XDB-C8, 4.6 mm x 150 mm, particle size 5 µm, the limit temperature is 60 ° C, pH = 2-9); flow rate: 1.5 mL/min; Column temperature: 27°C; injection volume: 10µL; 280 nm. For the drug, standard curve was linear from 0.5 to 9 µg/mL. The method was validated and applied on pharmaceutical dosage forms and on biological samples as well. It was obtained optimal results. Thus, it can be used in the control of synthetic process and determination of loratadine in drug substance, pharmaceutical preparation and biological samples.