ROXANA POPESCU, CRISTINA VLAD DALIBORCA, ADINELA CIMPORESCU, CRISTIAN S. VLAD, DOINA VERDES, DORICA BOTAU, MARIOARA N. FILIMON, IVAN PAULIUC, COSMIN CITU, VICTOR DUMITRASCU CHEMICAL PROPERTIES AND IN VITRO ANTITUMOR EFFECTS OF MOMORDICA CHARANTIA EXTRACTS IN DIFFERENT SOLVENTS
Momordica Charantia also known as bitter melon, is used in traditional medicine for its antidiabetic, anticancer and antiviral properties. It is known that a large number of pathological damage is caused by reactive oxygen species. Plant polyphenols with antioxidant capacity can neutralize these reactive chemical species preventing the damage of cellular functions. The aim of our study was to determine the antitumor effects, antioxidant activity, phenolic compounds, and protein concentrations of ethanol and aqueous extracts of M. charantia sprouts. The ethanol and aqueous extracts antiproliferative potential was observed in dose – dependent manner. We observed an increase of antioxidant activity in the case of ethanol extract showed by decrease of absorbance. In aqueous extract we did not found significant differences regarding antioxidant activity. The total phenolic content of both extracts increased proportionally with concentration. Analysis by Lowry method showed an increased content of proteins in both aqueous and ethanol extract compared to results obtained by Bradford assay. The study suggests that both extracts may be considered a source with bioactive properties which could be of interest for pharmaceutical use.
Keywords. Bitter melon extracts, antioxidant and antitumor activity, protein concentration