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REVISTA DE CHIMIE
Cite as: Rev. Chim.
https://doi.org/10.37358/Rev.Chim.1949

OSIM Nr. R102355
ISSN Online 2668-8212
ISSN Print: 1582-9049
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Revista de Chimie (Rev. Chim.), Year 2006, Volume 57, Issue 10,





Determination of Glyphosat
Herbicide and Its Main Metabolite, Amino-Methyl-Phosphonic Acid (AMPA) in Water Using HPLC


Abstract:

In this paper, we reported a comparative study about extraction and identification methods of glyphosat herbicide and its main metabolite amino-methyl-phosphonic acid (AMPA) present in water through direct derivation with 9-fluorenilmehtyl-cloroformiat (FMOCCl) and identification using HPLC with fluorescent and diode array detector (DAD). The calibration curve for the pesticides in range 0.00075-0.0125 µg/mL was linear and with high degree of reproducibility. The recovery rates and the efficiencies for the pesticides derivation from unionized water were calculated. The efficiencies for glyphosat derivation were less than in the case of metabolite AMPA derivation. The minimal limit for detection in the case of fluorescent detector was 0.047 µg glyphosat/l water and 0.0155 µg AMPA/l water, and for diode array detector (DAD) 0.3179 µg Glyphosat /l water respectively 0.1034 µg AMPA/l water. Keywords: glyphosate, AMPA, water, High-Performance Liquid Chromatography (HPLC).

Issue: 2006, Volume 57, Issue 10
Pages:
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This article is published under the Creative Commons Attribution 4.0 International License
 
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