ABOUT INDEXING BOARD ARCHIVES AUTHOR GUIDELINES SUBMIT PAPER AUTHOR'S PAGE NEWS, EVENTS CONTACT
REVISTA DE CHIMIE
Cite as: Rev. Chim.
https://doi.org/10.37358/Rev.Chim.1949

OSIM Nr. R102355
ISSN Print 0034-7752
ISSN Online 2668-8212
Journal Metrics
* Impact Factor 2019: 1.755
* 5-Year IF: 1.351
* CiteScore: 2.2
* H index: 28
(InCites Journal Citation Reports)

SPECIAL ISSUE >>>

REVISTA DE CHIMIE Latest Issue

LATEST ISSUE >>>

   Volume 71, 2020
   Volume 70, 2019
   Volume 69, 2018
   Volume 68, 2017
   Volume 67, 2016
   Volume 66, 2015
   Volume 65, 2014
   Volume 64, 2013
   Volume 63, 2012
   Volume 62, 2011
   Volume 61, 2010
   Volume 60, 2009
   Volume 59, 2008
   Volume 58, 2007
 
<<<< back

Revista de Chimie (Rev. Chim.), Year 2007, Volume 58, Issue 1,





Validation of a HPLC Method for Atenolol Determination in Human Plasma

Abstract:

An HPLC method with fluorescence detection for atenolol determination in human plasma was developed and validated, using metoclopramide as internal standard. After protein precipitation with perchloric acid, atenolol was separated at 30 °C on a C18 column, 150 mm x 4.6 mm, 5 µm. The mobile phases consisted of a mixture of acetonitrile:sodium dihydrogen phosphate 40 mM, pH 3 with phosphoric acid 85%, 10:90 (solvent A), and acetonitrile (solvent B). The elution was made in gradient mode: 0-4 min 0%B, 4-8 min 30% B, 8-13 min 0%B, at 1 ml/min. The excitation and emission wavelengths were Ex/Em = 235/ 315 nm for 7.5 min, and 309/356 until 13 min. The method was linear between 10-1000 ng/ml, with an accuracy and precision of -8.6¸6.3 %, being between -18.7% and 9.8% at lower limit of quantification. The method was applied for atenolol determination in human plasma after oral administration of 100 mg atenolol. Keywords: atenolol, HPLC, plasma, metoclopramide

Issue: 2007, Volume 58, Issue 1
Pages:
download pdf   Download Pdf Article
Creative Commons License
This article is published under the Creative Commons Attribution 4.0 International License
 
<<<< back
 
PUBLISHING SECTION
Author Guidelines
 * Paper Drafting
 * Paper Template
 * Ethical Statement
 * Paper Submission
 * Author Payments
Ghidul Autorului (ro)
Submit Paper
Author's Page
Publishing Ethics
Peer Review Process
Open Access & Archiving Policy
 Search Authors
 LATEST NEWS
REvista de Chimie Revista de Chimie - 70 de ani de aparitie neintrerupta.. detalii
dictionar English-Romanian Dictionary for Mechanical Engineering
(download pdf)
  News, Events
Crossref Member Badge
 DOI  logo
 Gold Open Access | Source=http://www.plos.org/  | Author=art designer at PLoS
Creative Commons License
 Conferences of Contemporary
 Chemistry
 Biblioteca Chimiei
 Materiale Plastice