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REVISTA DE CHIMIE
Cite as: Rev. Chim.
https://doi.org/10.37358/Rev.Chim.1949

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Revista de Chimie, Volume 59, Issue 1, pp. (2008)




Micellar Electrokinetic Capillary Chromatography of 1,4-Benzodiazepine Derivates and their Degradation Products

Abstract:

The aim of this work is to elaborate a fast and reliable capillary electrophoresis method, for the separation of 1,4-benzodiazepine (BZD) derivates and their degradation products in acidic medium and in UV light. Because BZDs are neutral, hydrophobic compounds, the most efficient method proved to be micellar elektrokinetic capillary chromatography (MECC). For the separation we used a sodium tetraborate buffer with tensioactive agent (sodium dodecyl sulphate - SDS) and an organic modifier (methanol). We also optimized the analytical conditions, and studied the effects of the SDS and methanol concentrations and buffer pH on the determination efficiency. Keywords: 1,4-benzodiazepines derivates, micellar elektrokinetic capillary chromatography, degradation products, separation

Issue: 2008 Volume 59, Number 1
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