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Revista de Chimie (Rev. Chim.), Year 2008, Volume 59, Issue 11,

https://doi.org/10.37358/RC.08.11.2014

Gheorghe Benga, Ioana-Costina Dânsoreanu, Maria Frangopol, Petre T. Frangopol

Some Applications of Spin Labels in the Study of Serum Albumin and Biomembranes

Abstract:

The main contributions of the groups lead by the two main authors (Gh.B and P.T.F.) in the field of spin labeling ESR in studies of serum albumin and of some biomembranes are briefly described, mentioning some priorities. These are: the description in Romanian publications of the methodology for using spin labels in studies of proteins and biomembranes using the type ART-6 ESR spectrometer manufactured in Romania; the adaptation to this ESR spectrometer of a computerized system for the acqusition and processing of data; the application of a newly synthesized spin label, reactive with SH groups, in the study of serum albumin; the finding of the effects of 25-hydroxycholesterol at low concentrations (0,5-10 %) on egg yolk lecitin; the finding of a relatively high fluidity of human liver microsomes.

Keywords:
electron spin resonance; spin labels; biomembranes; serum albumin; cytochrome oxidase; liposomes; microsomes; human liver; rat liver; phenotiozines; cholesterol

Issue: 2008, Volume 59, Issue 11
Pages:
Publication date: 2008/10/9
https://doi.org/10.37358/RC.08.11.2014
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