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Revista de Chimie (Rev. Chim.), Year 2009, Volume 60, Issue 10,





SILVIA IGA, ADRIAN IGA, MAGDALENA TÎRCOL, DUMITRU PETRU IGA
Physico-Chemical Proofs Concerning the Existence
of Ganglioside GM3 in Normal Human LDL

Abstract:

Low density lipoproteins (LDL) were separated from normal human serum by a standard procedure. Total lipids of LDL were recovered by chloroform-methanol extraction and submitted to Folch partition. Lipids from upper layer were dialysed, submitted to ion-exchange chromatography on DEAE-Sephadex A-25 and the fractions containig lipid bound sialic acid passed on a column of silica gel. Thin layer chromatography of crude mixture, in comparison with adequated standards suggested the presence of ganglioside GM3. A compound isolated from LDL lipids, by techniques currently used in gangliosides separation, produced a compound co-migrating with ganglioside GM3. Desialysation of this compound produced a glycolipid which co-chromatographed with standard lactosyl-ceramide. 1H NMR Spectra of the isolated compound indicated that it contained all the structural ingredients of ganglioside GM3: sialic acid, D-galactose, D-glucose, sphingosine, fatty acid. Concentration of lipid bound sialic acid in normal human LDL was 8.5 nmols sialic acid/mg of protein. Key words: ganglioside GM3, lactosyl-ceramide, low density lipoproteins, human serum, atherosclerosis


Issue: 2009, Volume 60, Issue 10
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