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REVISTA DE CHIMIE
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https://doi.org/10.37358/Rev.Chim.1949

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Revista de Chimie (Rev. Chim.), Year 2011, Volume 62, Issue 1,



Cezar Comanescu, Denisa Ficai, Ioana Lacatusu, Cornelia Guran

New Organic - Inorganic Hybrid Materials for Enzymes Immobilization

Abstract:

The purpose of this study is the preparation and characterization of organic-inorganic hybrid materials used for enzyme immobilization on silica-based support by cross-linking method. The cross-linking agent used was glutaraldehyde and the enzyme used for immobilization was lipase. Organic-inorganic hybrid materials were obtained by the sol-gel process in the presence of different surfactant systems. A nanosized silica-based support was synthesized and characterized by dynamic light scattering and scanning microscopy analysis. The modifications in the chemical composition of the organic-inorganic hybrid materials before and after lipase immobilization were analyzed by infrared spectroscopy. The amount of immobilized lipase (Lowry method) and the enzymatic activity were determined.
Keywords:
silica-based support; enzyme immobilization; lipase; enzymatic activity

Issue: 2011, Volume 62, Issue 1
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