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REVISTA DE CHIMIE
Cite as: Rev. Chim.
https://doi.org/10.37358/Rev.Chim.1949

OSIM Nr. R102355
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Revista de Chimie (Rev. Chim.), Year 2011, Volume 62, Issue 3,





Gheorghe Maria, Adela Cocuz
Operating Alternatives for the (semi)batch Reactor used
for D-glucose Enzymatic Oxidation with Free-enzymes

Abstract:

Batch and semi-batch reactors are very suitable for conducting enzymatic reactions within a wide range of applicative areas including food, pharmaceutical, textile industry, and biochemical synthesis. Free enzyme operation can be often preferred, by taking advantage of enzyme full activity leading to high yields. Such an alternative is suitable when the product separation does not raise special problems, or when its contamination with the enzyme is not very important. Optimal and flexible operation of such reactors are subjects of continue interest, due to the possibility of improving their productivity, with a considerable reduction in enzyme consumption. A “real life” example is provided for the case of a design industrial reactor used for D-glucose enzymatic oxidation in the rare sugar production lines. By investigating several operating alternatives, including simple batch, batch with intermittent addition of enzyme, or semi-batch with constant or optimal enzyme feeding policy, the study proves how considerable savings in enzyme consumption can be obtained without any productivity or conversion loss. Advantages and drawbacks of the checked operating alternatives are comparatively discussed to derive relevant conclusions of general use. Keywords: semi-batch reactor, optimization, D-glucose, oxidation, pyranose oxidase, catalase

Issue: 2011, Volume 62, Issue 3
Pages:
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