ERDAL DINÇ,  DUMITRU BALEANU,   EMRE  KÜÇÜKBAGRIAÇIK<br>Ultra Performance Liquid Chromatography for the Quantitative Analysis of Ampicillin Sodium and Sulbactam Sodium in a Pharmaceutical Preparation

ERDAL DINÇ, DUMITRU BALEANU, EMRE KÜÇÜKBAGRIAÇIK
Ultra Performance Liquid Chromatography for the Quantitative Analysis of Ampicillin Sodium and Sulbactam Sodium in a Pharmaceutical Preparation

Abstract:

In this article a new method based on ultra performance liquid chromatography (UPLC) was proposed for the simultaneous rapid quantitative analysis of of ampicillyn sodium (AS) and sulbactam sodium (SS) in a commercial preparation. Chromatographic separation and quantitation of allura red were performed on an Acquiting UPLCTM BEH Phenyl (100 mm x 1.0 mm, i.d., 1.7 mm ) column system and a mobile phase corresponding to a mixture cosisting of acetonitrile and 0.1 M (NH4)2CO3 (70:30, v/v) containing 2 mL of trietylamine at a constant flow rate, 1.0 mL/min. Chromatographic calibration graphs in the linear range of 10-80 mg/mL for SS and 10-70 mg/mL for AS was calculated by using the linear regression analysis based on the relationship between the concentration and the peak area with a detection at the wavelength 220 nm. The UPLC method was validated by using the synthetic mixtures and successfully applied to the UPLC quantitation of the commercial samples of the related drugs. We concluded that a good agreement of UPLC quantitation results was reported. Keywords: Ultra performance liquid chromatography, ampicillin sodium, sulbactam sodium

Issue: 2012, Volume 63, Issue 5

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